Comparison of urinary 6‐β‐cortisol and the erythromycin breath test as measures of hepatic P450IIIA (CYP3A) activity
Comparison of urinary 6‐β‐cortisol and the erythromycin breath test as measures of hepatic P450IIIA (CYP3A) activity
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尿 6-β-皮质醇与红霉素呼气试验作为肝 P450IIIA (CYP3A) 活性测量指标的比较
DOI：
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
J. Voorhees
中科院分区：
文献类型：
--
作者：
P. Watkins;D. Turgeon;Paul V. Saenger;K. Lown;J. Kolars;T. Hamilton;Kenneth Fishman;P. Guzelian;J. Voorhees
静脉注射剂量的[14C‐N‐甲基]‐红霉素（红霉素呼吸试验[ERMBT]）和尿液中6‐β‐皮质醇与游离皮质醇（6‐β‐F/FF）的比例的测量都被提出作为测量患者肝脏P450IIIA催化活性的方法。我们发现，在47名无肝脏疾病且未服用被认为影响P450IIIA催化活性的药物的患者中，各项检测结果之间存在显著相关性（r = 0.59, p < 0.001）。在24例随后接受P450IIIA底物环孢素治疗的患者中，ERMBT结果与观察到的环孢素平均谷血水平高度相关；然而，尿6 - β - F/FF与环孢素血药水平之间没有相关性。在另一项对肝移植手术无肝期患者的研究中，ERMBT结果下降了85%以上，而尿6 - β - F/FF仅下降了50%。我们得出结论，ERMBT和尿6 - β - F/FF并不总是提供关于患者P450IIIA催化活性的相似信息，可能是因为6 - β - F的肝外生成。在这两种试验中，ERMBT似乎为环孢素给药提供了最相关的信息。
The production of 14CO2 in the breath from an intravenous dose of [14C‐N‐methyl] ‐erythromycin (the erythromycin breath test [ERMBT]) and the measurement of the ratio of 6‐β‐cortisol to free cortisol (6‐β‐F/FF) in the urine have each been proposed as means of measuring hepatic P450IIIA catalytic activity in patients. We found that there was a significant correlation between the results of each test (r = 0.59, p < 0.001) in 47 patients who were without liver disease and who were not taking medications believed to influence P450IIIA catalytic activity. In the 24 of these patients who were subsequently treated with the P450IIIA substrate cyclosporine, the ERMBT result was highly correlated with the mean trough cyclosporine blood level observed; however, there was no correlation between urinary 6‐β‐F/FF and the cyclosporine blood levels. In a separate study of a patient during the anhepatic phase of liver transplantation surgery, the ERMBT result decreased by greater than 85%, whereas urinary 6‐β‐F/FF decreased by just 50%. We conclude that the ERMBT and urinary 6‐β‐F/FF do not always provide similar information about P450IIIA catalytic activity in patients, possibly because of extrahepatic production of 6‐β‐F. Of the two tests, the ERMBT appears to provide the most relevant information for cyclosporine administration.
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影响因子：
15.9
作者：
WATKINS, PB;WRIGHTON, SA;GUZELIAN, PS
通讯作者：
GUZELIAN, PS
DOI：
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发表时间：
1986-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
F. Guengerich;M. Martín;P. Beaune;P. Kremers;T. Wolff;D. Waxman
通讯作者：
F. Guengerich;M. Martín;P. Beaune;P. Kremers;T. Wolff;D. Waxman
影响因子：
3.9
作者：
WAXMAN, DJ;ATTISANO, C;LAPENSON, DP
通讯作者：
LAPENSON, DP
细胞色素 P450 3A 在两栖动物、大鼠和人肾中的表达。
影响因子：
3.9
作者：
Schuetz,EG;Schuetz,JD;Grogan,WM;Naray-Fejes-Toth,A;Fejes-Toth,G;Raucy,J;Guzelian,P;Gionela,K;Watlington,CO
通讯作者：
Watlington,CO
人肝脏中细胞色素 P-450 诱导形式的鉴定。
DOI：
10.1073/pnas.82.18.6310
发表时间：
1985
影响因子：
11.1
作者：
Watkins,PB;Wrighton,SA;Maurel,P;Schuetz,EG;Mendez-Picon,G;Parker,GA;Guzelian,PS
通讯作者：
Guzelian,PS