ELECTROPHYSIOLOGICAL STUDY OF DORSAL RESPIRATORY NEURONS IN THE MEDULLA-OBLONGATA OF THE RAT
ELECTROPHYSIOLOGICAL STUDY OF DORSAL RESPIRATORY NEURONS IN THE MEDULLA-OBLONGATA OF THE RAT
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DOI：
10.1016/0006-8993(94)91763-9
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发表时间：
1994-03-07
期刊：
影响因子：
2.9
通讯作者：
KANJHAN, R
中科院分区：
文献类型：
--
作者：
DECASTRO, D;LIPSKI, J;KANJHAN, R
一直有争议的背侧呼吸组（DRG），确定在猫和其他几个物种的集中，主要是吸气神经元位于孤束腹外侧亚核，也存在于大鼠。本研究的目的是重新审视这一问题，系统地探索这一地区与细胞外微电极，在麻醉和人工通气大鼠。记录了142个单位，这些单位与中枢呼吸周期（通过膈神经记录确定）和/或肺充气同步击发。119个记录被认为来自神经元细胞体（在某些情况下通过对DL-同型半胱氨酸微电泳给药的兴奋性反应证实），而其余23个来自肺迷走神经传入。前一组的大多数神经元（87/119）是吸气神经元。在96个脊髓投射神经元中，只有14个（12个吸气，2个呼气）在刺激C-3段后产生逆向反应。这些结果证实了大鼠背根神经节的存在，并表明位于该区域的神经元具有与先前在猫中描述的那些基本相似的放电模式。主要差异是大鼠中在C-2水平以下脊髓投射的神经元相对缺乏，这表明该物种中的大多数背根神经节神经元不直接投射到膈和肋间运动神经元，而是投射到其他，尚未鉴定的脑干或上颈段内的神经元群。
There has been controversy whether the dorsal respiratory group (DRG), identified in the cat and several other species as a concentration of mainly inspiratory neurons located in the ventrolateral subnucleus of the solitary tract, also exists in the rat. The aim of this study was to re-examine this question by systematically exploring this region with extracellular microelectrodes, in anesthetized and artificially ventilated rats. One-hundred and forty-two units were recorded which fired in phase with central respiratory cycles (determined by recording from the phrenic nerve) and/or lung inflations. One-hundred and nineteen recordings were thought to be from neuronal cell bodies (confirmed in some cases by excitatory responses to microelectrophoretic administration of DL-homocysteic acid), while the remaining 23 were from lung vagal afferents. Most neurons in the former group (87/119) were inspiratory. Out of 96 neurons tested for spinal projections only 14 (12 inspiratory, 2 expiratory) responded antidromically following stimulation at C-3 segment. These results confirm the existence of the DRG in the rat and demonstrate that neurons located in this region have firing patterns generally similar to those previously described in the cat. The main difference is the relative paucity in the rat of neurons projecting spinally below the C-2 level, which indicates that most DRG neurons in this species do not project directly to phrenic and intercostal motoneurons, but to other, as yet unidentified, neuronal groups within the brainstem or upper cervical segments.