Initial steps in lymph node metastasis formation in an experimental system: possible involvement of recognition by macrophage C-type lectins
Initial steps in lymph node metastasis formation in an experimental system: possible involvement of recognition by macrophage C-type lectins
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DOI：
10.1007/s002620050021
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发表时间：
2000-04-01
影响因子：
5.8
通讯作者：
Irimura, T
中科院分区：
文献类型：
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作者：
Ichii, S;Imai, Y;Irimura, T
我们使用组织学观察和荧光细胞示踪剂实验来研究组织巨噬细胞通过半乳糖/N-乙酰半乳糖胺特异性C型凝集素（mMGL）识别在小鼠卵巢肿瘤OV2944-HM-1（HM-1）细胞淋巴结转移形成中的作用。皮下部位的淋巴结转移是由肿瘤细胞进入 mMGL 阳性细胞主要位于的淋巴结被膜下窦引发的。为了研究 mMGL 阳性细胞是否有助于宿主抵抗淋巴结转移，我们用 mMGL 阻断单克隆抗体反复治疗携带移植肿瘤的小鼠，该抗体已知可抑制 mMGL 与其配体的结合。当用阻断性抗 mMGL 抗体治疗小鼠时，皮下注射 2 周后从淋巴结回收的 HM-1 细胞数量显着增加。这些结果表明 mMGL 阳性巨噬细胞有助于宿主防御淋巴结转移。
We used histological observations and experiments with fluorescent cell tracers to investigate the roles of tissue macrophages in recognition through a galactose/N-acetylgalactosamine-specific C-type lectin (mMGL) in lymph node metastasis formation by mouse ovarian tumor OV2944-HM-1 (HM-1) cells. Lymph node metastasis from subcutaneous sites was shown to be initiated by the entry of tumor cells into the subcapsular sinus of lymph nodes where mMGL-positive cells were mainly located. To investigate whether mMGL-positive cells contributed to host resistance against lymph node metastasis, we repeatedly treated mice bearing transplanted tumors with an mMGL-blocking monoclonal antibody that was known to inhibit mMGL binding to its ligands. The number of HM-1 cells recovered from lymph nodes 2 weeks after subcutaneous injections was significantly greater when the mice were treated with the blocking anti-mMGL antibody. These results suggested that mMGL-positive macrophages contributed to the host's defense against lymph node metastasis.