Systemic acyl-ghrelin increases tail skin temperature in rats without affecting their thermoregulatory behavior in a cold environment.
Systemic acyl-ghrelin increases tail skin temperature in rats without affecting their thermoregulatory behavior in a cold environment.
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全身性酰基生长素释放肽可增加大鼠尾部皮肤温度，而不影响其在寒冷环境中的体温调节行为。
DOI：
10.1016/j.neulet.2020.135306
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发表时间：
2020
影响因子：
2.5
通讯作者：
Izumi Sato
中科院分区：
文献类型：
--
作者：
Yuki Uchida;Chinami Tsunekawa;Izumi Sato
禁食会增加Ghrelin，这是一种具有两种循环异构体的多肽荷尔蒙，即酰基和去酰基Ghrelin。我们报道了禁食或去酰基Ghrelin促进大鼠在寒冷中的行为温度调节，通过隐藏尾巴行为来评估，这是大鼠在寒冷中体温调节行为的指标；然而，酰基Ghrelin对相同过程的影响仍有待阐明。我们研究了酰基-Ghrelin对大鼠寒冷时体温调节行为的影响。动物接受腹腔注射生理盐水或24 μg 酰基-Ghrelin，在27 °C或15 °C暴露2 h，同时持续监测它们的体温、尾部皮肤温度和藏尾行为。计数正中视前区、内侧视前区、室旁核(PVN)和弓状核的CFOS免疫反应细胞(CFOS-IR)。在15 °C下，酰基生长激素处理组的体温和体温调节行为持续时间与对照组无显著差异，但尾部皮肤温度高于对照组。27 °C时，对照组下丘脑室旁核内cFos-IR细胞数明显多于乙酰基-Ghrelin干预组。这些结果表明，在寒冷条件下，酰基-Ghrelin不影响大鼠行为体温调节，但可能影响尾部皮肤温度。
Fasting increases ghrelin that is a peptide hormone with two circulating isoforms, acyl and des-acyl ghrelin. We reported that fasting or des-acyl ghrelin facilitates behavioral thermoregulation in the cold in rats assessed by tail-hiding behavior that was the indicator of rats’ thermoregulatory behavior in the cold; however, the effect of acyl-ghrelin on the same process remains to be elucidated. We investigated the effect of acyl-ghrelin on thermoregulatory behavior in the cold in rats. The animals received an intraperitoneal saline or 24 μg acyl-ghrelin injection and were exposed to 27 °C or 15 °C for 2 h, while their body temperature, tail skin temperature, and tail-hiding behavior were constantly monitored. cFos immunoreactive (cFos-IR) cells in the median preoptic area, medial preoptic area, paraventricular nucleus (PVN), and arcuate nucleus were counted. Body temperature and the duration of thermoregulatory behavior did not show a significant difference between the acyl-ghrelin-treated and control groups at 15 °C; however, tail skin temperature in the acyl-ghrelin-treated group was higher than that in the control group. The number of cFos-IR cells in the PVN was greater in the control group than that in the acyl-ghrelin-treated group at 27 °C. These results indicate that acyl-ghrelin did not affect behavioral thermoregulation but might affect tail skin temperature in rats in the cold.