THE SENSITIVITY OF EXPERIMENTS WHICH USE GRADIENT PULSES FOR COHERENCE-PATHWAY SELECTION
THE SENSITIVITY OF EXPERIMENTS WHICH USE GRADIENT PULSES FOR COHERENCE-PATHWAY SELECTION
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DOI：
10.1006/jmra.1994.1227
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发表时间：
1994-11-01
期刊：
影响因子：
--
通讯作者：
KEELER, J
中科院分区：
文献类型：
--
作者：
KONTAXIS, G;STONEHOUSE, J;KEELER, J
在多维NMR实验中使用场梯度脉冲进行相干性选择，与使用相位循环的实验相比，可能会导致每单位时间灵敏度的降低。此外，必须注意以这样的方式引入梯度，以保持产生具有纯相的光谱的可能性（即，吸收模式线形）。针对这些问题，对多维实验中常用的实现梯度脉冲的方法进行了评价和比较。这些预测证实了一个简单的分子系统。实验实现的灵敏度进行了比较，在残基的基础上，不同的氮-15质子HSQC光谱的166个残基的蛋白质的情况下。(C)1994年出版社出版。
The use of field-gradient pulses for coherence selection in multidimensional NMR experiments may cause a reduction in sensitivity per unit time when compared to experiments which use phase cycling. In addition, care must be taken to introduce the gradients in such a way as to retain the possibility of producing spectra with pure phase (i.e., absorption-mode lineshapes). The commonly used methods of implementing gradient pulses in multidimensional experiments are evaluated and compared with respect to these issues. These predictions are confirmed for a simple molecular system. The experimentally achieved sensitivities are also compared, on a residue-by-residue basis, for the case of different nitrogen-15 proton HSQC spectra of a 166 residue protein. (C) 1994 Academic Press, Inc.