Age-dependent retinoic acid regulation of gene expression distinguishes the cervical, thoracic, lumbar, and sacral spinal cord regions during development.
Age-dependent retinoic acid regulation of gene expression distinguishes the cervical, thoracic, lumbar, and sacral spinal cord regions during development.
复制标题
视黄酸对基因表达的年龄依赖性调节可区分发育过程中的颈椎、胸椎、腰椎和骶椎脊髓区域。
DOI：
10.1159/000017373
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发表时间：
1999
影响因子：
2.9
通讯作者：
LaMantia,AS
中科院分区：
文献类型：
--
作者：
Rubin,WW;LaMantia,AS
我们评估是否在视黄酸（RA）的可用性的差异建立不同的模式，RA依赖的基因表达在胚胎小鼠胸/骶与颈/腰脊髓区域。外源性RA诱发RA激活的转基因和RA受体β在小鼠胚胎第12.5天（E），但不是E14.5背胸和骶髓异位表达。这种年龄依赖性调节是细胞自主的，并且不伴随着几种类维生素A受体、结合蛋白或SMRT核辅阻遏物的表达模式的变化。相反，这种变化显然反映了E12.5和E14.5之间胸背和骶髓内源性RA的丢失。因此，长期暴露于外源性RA之间E11.5和E13.5恢复异位RA介导的基因表达。这些观察结果表明，当地的可用性RA建立绝对差异的基因表达，区分胸，骶髓颈，腰髓在妊娠中期。
We evaluated whether differences in the availability of retinoic acid (RA) establish distinct patterns of RA-dependent gene expresion in the embryonic mouse thoracic/sacral versus cervical/lumbar spinal cord regions. Exogenous RA elicits ectopic expression of an RA-activated transgene and the RA receptor beta in the dorsal thoracic and sacral cord in mice at embryonic day (E) 12.5, but not E14.5. This age-dependent regulation is cell autonomous and is not accompanied by changes in expression patterns of several retinoid receptors, binding proteins, or the SMRT nuclear corepressor. Instead, this change apparently reflects the loss of endogenous RA in the dorsal thoracic and sacral cord between E12.5 and E14.5. Thus, chronic exposure to exogenous RA between E11.5 and E13.5 restores ectopic RA-mediated gene expression. These observations suggest that the local availability of RA establishes absolute differences in gene expression that distinguish the thoracic and sacral cord from the cervical and lumbar cord during midgestation.