Proteome survey reveals modularity of the yeast cell machinery
Proteome survey reveals modularity of the yeast cell machinery
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DOI：
10.1038/nature04532
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发表时间：
2006-03-30
期刊：
影响因子：
64.8
通讯作者：
Superti-Furga, G
中科院分区：
文献类型：
--
作者：
Gavin, AC;Aloy, P;Superti-Furga, G
蛋白质复合物是整合多种基因产物以执行细胞功能的关键分子实体。在这里，我们报告了第一个全基因组筛选复合物的生物体，芽殖酵母，使用亲和纯化和质谱。通过系统标记开放阅读框（ORF），大多数复合物被纯化数次，表明筛选饱和。丰富的数据集使从头表征的组成和组织的细胞机器。细胞蛋白质的集合分为491个复合物，其中257个是新的，其与另外的附着蛋白或蛋白质模块差异性地联合收割机组合，以使潜在功能多样化。蛋白质组的这种模块化组织的支持来自与表达，定位，功能，进化保守，蛋白质结构和二元相互作用的可用数据的整合。这项研究提供了迄今为止最大的物理确定的真核细胞机器的集合，以及生物数据集成和建模的平台。
Protein complexes are key molecular entities that integrate multiple gene products to perform cellular functions. Here we report the first genome-wide screen for complexes in an organism, budding yeast, using affinity purification and mass spectrometry. Through systematic tagging of open reading frames (ORFs), the majority of complexes were purified several times, suggesting screen saturation. The richness of the data set enabled a de novo characterization of the composition and organization of the cellular machinery. The ensemble of cellular proteins partitions into 491 complexes, of which 257 are novel, that differentially combine with additional attachment proteins or protein modules to enable a diversification of potential functions. Support for this modular organization of the proteome comes from integration with available data on expression, localization, function, evolutionary conservation, protein structure and binary interactions. This study provides the largest collection of physically determined eukaryotic cellular machines so far and a platform for biological data integration and modelling.