Proteomic analysis of early germs with high-oil and normal inbred lines in maize
Proteomic analysis of early germs with high-oil and normal inbred lines in maize
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DOI：
10.1007/s11033-008-9250-3
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发表时间：
2009-04-01
影响因子：
2.8
通讯作者：
Li, Jiansheng
中科院分区：
文献类型：
--
作者：
Liu, Zhanji;Yang, Xiaohong;Li, Jiansheng
高油玉米作为长期选择的产物，为功能基因组学研究提供了独特的资源。本研究采用双向凝胶电泳结合质谱技术，比较了高油玉米和普通玉米早期胚发育过程中可溶性蛋白的含量。用银染法在高油系和正常系的电泳图谱上检测到1100多个蛋白质点。在高油自交系与正常自交系之间，共有83个蛋白质点存在显著差异表达（> 2倍变化; t检验：P < 0.05）。基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）鉴定了27个蛋白质点，其中包括25个非冗余蛋白。这些蛋白质的功能分类为碳水化合物代谢、细胞骨架、蛋白质代谢、应激反应和脂质代谢。三个这样的蛋白质参与脂质代谢，即推定的烯酰-ACP还原酶（ENR），推定的硬脂酰-ACP去饱和酶（SAD）和推定的乙酰辅酶A C-酰基转移酶（ACA），有更丰富的高油系比在正常的表达。在mRNA表达水平上，SAD、ENR和ACA在高油系中的表达水平显著高于正常油系。结果表明，SAD、ENR和ACA的高表达可能与高油玉米中油浓度的增加有关。本研究首次对高油玉米进行了蛋白质组学分析，有助于更好地了解高油玉米油分积累的分子基础。
High-oil maize as a product of long-term selection provides a unique resource for functional genomics. In this study, the abundant soluble proteins of early developing germs from high-oil and normal lines of maize were compared using two-dimensional gel electrophoresis (2-DGE) in combination with mass spectrometry (MS). More than 1100 protein spots were detected on electrophoresis maps of both high-oil and normal lines by using silver staining method. A total of 83 protein spots showed significant differential expression (> two-fold change; t-test: P < 0.05) between high-oil and normal inbred lines. Twenty-seven protein spots including 25 non-redundant proteins were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS). Functional categorization of these proteins was carbohydrate metabolism, cytoskeleton, protein metabolism, stress response, and lipid metabolism. Three such proteins involved in lipid metabolism, namely putative enoyl-ACP reductase (ENR), putative stearoyl-ACP desaturase (SAD) and putative acetyl-CoA C-acyltransferase (ACA), had more abundant expressions in high-oil lines than in normal. At the mRNA expression level, SAD, ENR and ACA were expressed at significantly higher levels in high-oil lines than in normal. The results demonstrated that high expressions of SAD, ENR and ACA might be associated to increasing oil concentration in high-oil maize. This study represents the first proteomic analysis of high-oil maize and contributes to a better understanding of the molecular basis of oil accumulation in high-oil maize.