New group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge Polypedilum vanderplanki
New group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge Polypedilum vanderplanki
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一组新的跨膜蛋白与脱水蠓 Polypedilum vanderplanki 的干燥耐受性相关
DOI：
10.1038/s41598-020-68330-6
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发表时间：
2020
影响因子：
4.6
通讯作者：
Shagimardanova Elena I.
中科院分区：
文献类型：
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作者：
Voronina Taisiya A.;Nesmelov Alexander A.;Kondratyeva Sabina A.;Deviatiiarov Ruslan M.;Miyata Yugo;Tokumoto Shoko;Cornette Richard;Gusev Oleg A.;Kikawada Takahiro;Shagimardanova Elena I.
沉睡的摇蚊的幼虫以其非凡的能力而闻名，它们在完全干燥的非代谢状态下存活下来，这种状态被称为“无水生物”。独一无二的OFP功能。浮游生物基因组是一种与脱水相关的扩增基因簇的存在。虽然几个这样的簇代表了已知基因的同源基因，但有一组不同的基因是P所特有的。范德普兰基。这些基因包括Lea-Island-Located(LIL)基因，除了两个LEA基因OFP外，没有已知的同源基因。范德普兰基、PvLea1和PvLea3。然而，PvLIL蛋白缺乏LEA的典型特征，如固有的无序状态、亲水性和特征的LEA4基序。它们每个都有四到五个跨膜结构域，我们证实了三个PvLIL的膜靶向性。PvLIL中的保守氨基酸位于跨膜区或其附近。PvLEA1和PvLEA3蛋白是结合了LEA样蛋白和跨膜区的嵌合体，与PvLIL蛋白相同。我们发现，在无水生物诱导过程中，PvLilgene在两种幼虫中都高度上调。范德普兰基和P。范德普兰基来源的培养细胞系Pv11。因此，PvLIL是一组新的有趣的基因，可能与无水生物有关，因为它们与一些LEA基因有共同的起源，并且它们在无水生物过程中被诱导。
Larvae of the sleeping chironomidPolypedilum vanderplankiare known for their extraordinary ability to survive complete desiccation in an ametabolic state called “anhydrobiosis”. The unique feature ofP. vanderplankigenome is the presence of expanded gene clusters associated with anhydrobiosis. While several such clusters represent orthologues of known genes, there is a distinct set of genes unique forP. vanderplanki. These include Lea-Island-Located (LIL) genes with no known orthologues except two of LEA genes ofP. vanderplanki,PvLea1andPvLea3. However, PvLIL proteins lack typical features of LEA such as the state of intrinsic disorder, hydrophilicity and characteristic LEA_4 motif. They possess four to five transmembrane domains each and we confirmed membrane targeting for three PvLILs. Conserved amino acids in PvLIL are located in transmembrane domains or nearby. PvLEA1 and PvLEA3 proteins are chimeras combining LEA-like parts and transmembrane domains, shared with PvLIL proteins. We have found thatPvLilgenes are highly upregulated during anhydrobiosis induction both in larvae ofP. vanderplankiandP. vanderplanki-derived cultured cell line, Pv11. Thus,PvLilare a new intriguing group of genes that are likely to be associated with anhydrobiosis due to their common origin with some LEA genes and their induction during anhydrobiosis.