ARGON ION LASER-EXCITED AUTOFLUORESCENCE IN NORMAL AND ATHEROSCLEROTIC AORTA AND CORONARY-ARTERIES - MORPHOLOGIC STUDIES
ARGON ION LASER-EXCITED AUTOFLUORESCENCE IN NORMAL AND ATHEROSCLEROTIC AORTA AND CORONARY-ARTERIES - MORPHOLOGIC STUDIES
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DOI：
10.1016/0002-8703(89)90239-1
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发表时间：
1989-11-01
影响因子：
4.8
通讯作者：
KRAMER, JR
中科院分区：
文献类型：
--
作者：
FITZMAURICE, M;BORDAGARAY, JO;KRAMER, JR
用476 nm氩离子激光照射的荧光显微镜对47例正常和动脉粥样硬化的人动脉和冠状动脉的未染色冰冻切片进行了氩离子激光激发的自体荧光研究，并与H & E、Movat pentachrome和油红O染色的连续切片的形态学进行了比较。正常动脉自发荧光与内膜、中膜和外膜中的结构蛋白纤维、弹性蛋白和胶原蛋白在形态学上相关。动脉粥样硬化斑块自发荧光与粥样硬化核心的脂质或钙化沉积物在形态学上相关。这些沉积物的自发荧光在分布、强度和颜色上不同于弹性蛋白和胶原，并且随着斑块的严重程度而增加。我们的结论是氩离子激光激发的自体荧光在正常和动脉粥样硬化的动脉形态学相关，并在激光血管外科诊断潜力。
Argon ion laser-excited autofluorescence was studied in unstained frozen sections of 47 normal and atherosclerotic human aortas and coronary arteries by means of a bright-field microscope modified for fluorescence microscopy with 476 nm argon ion laser epillumination, and compared to morphology in serial sections stained with H & E, Movat pentachrome, and oil red O. Normal artery autofluorescence correlated morphologically with the structural protein fibers elastin and collagen in the intima, media, and adventitia. Atherosclerotic plaque autofluorescence correlated morphologically with lipid or calcified deposits in the atheroma core. The autofluorescence of these deposits differed from that of elastin and collagen in distribution, intensity, and color, and increased with the severity of the plaque. We conclude that argon ion laser-excited autofluorescence in normal and atherosclerotic arteries correlates with morphology and has diagnostic potential in laser angiosurgery.