A primary study of the subgroups of T lymphocytes in MHV-3 induced chronic viral hepatitis
A primary study of the subgroups of T lymphocytes in MHV-3 induced chronic viral hepatitis
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DOI：
10.1007/s12250-007-0030-3
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发表时间：
2007-10
影响因子：
5.5
通讯作者：
Jiang-guo Zhang;Xiaomin Qin;Xiaojing Wang;W. Yan;Chuan-long Zhu;Xiaoping Luo;Q. Ning
中科院分区：
文献类型：
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作者：
Jiang-guo Zhang;Xiaomin Qin;Xiaojing Wang;W. Yan;Chuan-long Zhu;Xiaoping Luo;Q. Ning
为探讨T细胞亚群在小鼠肝炎病毒3型（MHV-3）诱导的C3 H/Hej小鼠慢性病毒性肝炎发病机制中的作用，选择90只C3 H/Hej小鼠，分别腹腔注射10个空斑形成单位（PFU）的MHV-3。从感染后第2天开始，采用标准空斑法和HE染色法检测肝组织病毒滴度和病理学变化。于感染后0、2、4、6、8、10、12、15、20、25、30、40 d，采用流式细胞术检测外周血、脾、肝组织中T细胞亚群（CD 3 + CD 4 + CD 8 −、CD 3 + CD 4 − CD 8+、CD 3 + CD 4 − CD 8 −、CD 3 + CD 4 + CD 25+、CD 3 + CD 4 + CD 25 −和CD 3 + CD 4 − CD 25+）占总T细胞的比例。我们观察到MHV-3感染后2天，C3 H/Hej小鼠的病毒滴度升高，并显示持续的病毒复制和肝脏炎性变化。在C3 H/Hej小鼠中，从MHV-3感染后2天起，双阴性T细胞（DN Treg细胞）和CD 4 + CD 25 +T细胞比率显著增加，而CD 3 + CD 4 + CD 8 −、CD 3 + CD 4 − CD 8+、CD 3 + CD 4 + CD 25 −和CD 3 + CD 4 − CD 25 + T细胞比率相应降低。总之，MHV-3后C3 H/Hej小鼠肝脏中病毒滴度和病理学的变化表明它们对病毒持续存在有贡献。DN Treg细胞的进一步特征是感染表明MHV-3可以诱导C3 H/Hej小鼠肝脏的慢性炎症。C3 H/Hej小鼠感染MHV-3后，DN Treg细胞和CD 4 + CD 25 +T细胞比例升高，提示DN Treg细胞和CD 4 + CD 25 +T细胞在慢性病毒性肝炎的发生发展中均具有重要的免疫抑制调节作用，在病毒持续感染中起重要作用。DNT细胞和CD 4 + CD 25 +T细胞的进一步特征正在研究中。
To study the contribution of T cell subsets in the pathogenesis of Murine hepatitis virus Type 3 (MHV-3) induced chronic viral hepatitis in C3H/Hej mice, ninety C3H/Hej mice were chosen to individually receive 10 plaque forming units (PFU) of MHV-3 intraperitoneally. The changes of virus titer and pathology in liver tissue were examined by standard plaque assay and by the hematoxylin/eosin (HE) staining method from 2 days post MHV-3 infection. The ratios of T cell subsets including CD3+CD4+CD8−, CD3+CD4−CD8+, CD3+CD4−CD8−, CD3+CD4+CD25+, CD3+CD4+CD25−and CD3+CD4−CD25+T lymphocyte of total T lymphocytes in blood, spleen and liver were examined at 0, 2, 4, 6, 8, 10, 12, 15, 20, 25, 30, 40 days post MHV-3 infection by flow cytosorting. We observed that the virus titer raised and showed persistent virus duplications and inflammatory changes in the livers of C3H/Hej mice from 2 days post MHV-3 infection. The double negative T cell (DN Treg cell) and CD4+CD25+T cell ratios increased significantly from 2 days post MHV-3 infection in C3H/Hej mice, and CD3+CD4+CD8−, CD3+CD4−CD8+, CD3+CD4+CD25−and CD3+CD4−CD25+T cell ratios decreased accordingly. In conclusion, the changes of virus titer and pathology in the livers of C3H/Hej mice post MHV-3 suggest their contribution to viral persistence. Further characterizations of DN Treg cells are that infection indicates that MHV-3 could induce the chronic inflammation in livers of C3H/Hej mice. The increase of the DN Treg cell and CD4+CD25+T cell ratios in C3H/Hej mice post MHV-3 infection suggests that DN Treg cells and CD4+CD25+T cells may both have important suppressive immunomodulation functions in the development of chronic viral hepatitis and have important roles in the virus persistent infection. Further characterizations of DNT cell and CD4+CD25+T cell are under investigation.