A 9 Å single particle reconstruction from CCD captured images on a 200 kV electron cryomicroscope
A 9 Å single particle reconstruction from CCD captured images on a 200 kV electron cryomicroscope
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DOI：
10.1016/j.jsb.2004.02.004
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发表时间：
2004-08-01
影响因子：
3
通讯作者：
Chiu, W
中科院分区：
文献类型：
--
作者：
Booth, CR;Jiang, W;Chiu, W
亚纳米分辨率结构的确定已成为大分子组件的电子冷冻显微镜检查中的一种常见实践。这些研究的数据迄今已在摄影膜上收集。使用细胞质多层病毒（CPV），一种先前确定的结构，作为测试标本，我们显示了从200 kV电子冰冻机镜上从4K x 4k Gatan CCD中获得的9 A结构的可行性。 CPV的蛋白质成分与同一病毒的先前结构中的alpha螺旋匹配证实了这种类型的摄像机的适用性，作为针对亚纳米分辨率下单个粒子重建的记录介质。 （c）2004 Elsevier Inc.保留所有权利。
Sub-nanometer resolution structure determination is becoming a common practice in electron cryomicroscopy of macromolecular assemblies. The data for these studies have until now been collected on photographic film. Using cytoplasmic polyhedrosis virus (CPV), a previously determined structure, as a test specimen, we show the feasibility of obtaining a 9 A structure from images acquired from a 4k x 4k Gatan CCD on a 200 kV electron cryomicroscope. The match of the alpha-helices in the protein components of the CPV with the previous structure of the same virus validates the suitability of this type of camera as the recording media targeted for single particle reconstructions at sub-nanometer resolution. (C) 2004 Elsevier Inc. All rights reserved.