Metabolomics profiling during biofilm development of Bacillus licheniformis isolated from milk powder
Metabolomics profiling during biofilm development of Bacillus licheniformis isolated from milk powder
复制标题
从奶粉中分离的地衣芽孢杆菌生物膜发育过程中的代谢组学分析
DOI：
10.1016/j.ijfoodmicro.2020.108939
复制
发表时间：
2021
影响因子：
5.4
通讯作者：
He Guoqing
中科院分区：
文献类型：
--
作者：
Wang Ni;Gao Jie;Yuan Lei;Jin Yujie;He Guoqing
地衣芽孢杆菌是乳品工业的主要微生物污染源，其生物膜的形成加剧了乳品安全问题。特别是对于奶粉制造商，喷雾干燥前在50 °C至70 °C之间的温度下的蒸发过程是防止嗜热菌繁殖的关键控制点。本研究采用代谢组学方法研究了细菌生物膜形成过程中代谢产物的动态变化及其作用。地衣化55 ℃ 24 h。氨基酸代谢十分活跃，脂肪代谢、碳水化合物代谢和核苷酸代谢相互配合。氨基酸生物合成提供了显着的贡献，特别是在早期生物膜的发展，从8至12小时。代谢产物参与精氨酸生物合成、半乳糖代谢和鞘脂代谢等特定途径，在生物膜的形成中起着至关重要的作用。这项工作提供了新的见解动态代谢交替和一个全面的网络在B。地衣形芽孢杆菌生物膜形成的代谢过程，这将扩大对生物膜形成过程的认识。地衣状。研究结果有助于改善乳品加工环境卫生，为保证乳品质量提供新的策略。
Bacillus licheniformis is a major source of microbial contamination to dairy industry, and biofilm formation by this spoilage bacterium aggravates the safety issues. Especially for milk powder manufactures, the evaporation process at temperatures between 50 °C and 70 °C before spray drying, is a critical control point against thermophilic bacteria multiplication. In our study, metabolomics analysis was performed to investigate dynamic changes of the metabolites and their roles during process of biofilm development ofB. licheniformisat 55 °C for 24 h. Amino acid metabolism was quite active, with cooperation from lipid metabolism, carbohydrate metabolism and nucleotide metabolism. Amino acid biosynthesis provided significant contributions especially during early biofilm development from 8 to 12 h. Metabolites involved in specific pathways of arginine biosynthetic, galactose metabolism and sphingolipid metabolism played a crucial role in building biofilm. This work provided new insights into dynamic metabolic alternations and a comprehensive network duringB. licheniformisbiofilm development, which will extend the knowledge on the metabolic process of biofilm formation byB. licheniformis. The results are helpful in creating better environmental hygiene in dairy processing and new strategies for ensuring quality of dairy products.