Additional products of the Tla locus of the mouse.
Additional products of the Tla locus of the mouse.
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小鼠 Tla 基因座的附加产物。
DOI：
10.1073/pnas.82.20.7044
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发表时间：
1985
影响因子：
11.1
通讯作者：
Boyse,EA
中科院分区：
文献类型：
--
作者：
Chorney,MJ;Shen,FW;Tung,JS;Boyse,EA
一些小鼠品系的胸腺细胞和白血病细胞产生 TL 蛋白，可通过抗 TL 抗血清和抗 TL 单克隆抗体沉淀，其中不仅包括熟悉的 45-50 kDa 重 (H) 链，还包括更高分子量的产物。详细研究了 Tlad 胸腺细胞产生 53-kDa TL 形式。 B10.M (Tlad) 小鼠（产生 53-kDa TL）与 A 品系（Tlaa）小鼠（仅产生通常的 H 链）杂交。在 Tlad/Tlaa 杂合子 F1 后代的胸腺细胞中观察到明显未改变的 53-kDa TL 的半表达。因此，没有迹象表明与 Tlad 相关的 53-kDa TL 形式的产生存在正反式相互作用或负反式相互作用。我们得出结论，53-kDa TL 的产生是由染色体内控制的。 Tlad 胸腺细胞的 TL H 链和 53-kDa TL 的二维胰凝乳肽图谱的不同之处仅在于 53-kDa TL 图谱中发现的附加特征。除 Tlaa 外，所有 Tla 等位基因 (Tlab-f) 都产生比伴随的 H 链分子量更高的 TL 产物，尽管在 Tlab 的情况下，这仅在 TL+ 白血病细胞中明显，因为 Tlab 胸腺细胞是 TL-。对于代表其他 I 类基因的 H-2，除了熟悉的 H 链之外，在类似条件下没有显示出任何产物。
Thymocytes and leukemia cells of some mouse strains yield TL proteins, precipitable by anti-TL antiserum and by anti-TL monoclonal antibodies, that include not only the familiar heavy (H) chain of 45-50 kDa but also products of higher molecular mass. Production of a 53-kDa TL form by Tlad thymocytes was studied in detail. A cross was made between B10.M (Tlad) mice, which produce the 53-kDa TL, and mice of the A strain (Tlaa), which make only the usual H chain. Hemi-expression of apparently unaltered 53-kDa TL was observed in thymocytes of the Tlad/Tlaa heterozygous F1 progeny. Thus, there was no indication of positive or negative trans interaction with respect to production of the 53-kDa TL form associated with Tlad. We conclude that production of 53-kDa TL is governed intrachromosomally. Two-dimensional chymotryptic peptide maps of the TL H chain and the 53-kDa TL of Tlad thymocytes differed only by added features found in the map of the 53-kDa TL. With the exception of Tlaa, all Tla alleles (Tlab-f) yielded TL products of higher molecular weight than the accompanying H chain, although in the case of Tlab this was evident only in TL+ leukemia cells because Tlab thymocytes are TL-. For H-2, representing other class I genes, no products other than the familiar H chain were demonstrable under similar conditions.