RAPID SCREENING OF URINE FOR SIGNIFICANT BACTERIURIA BY GRAM STAIN, ACRIDINE-ORANGE STAIN, AND THE AUTOBAC MTS SYSTEM
RAPID SCREENING OF URINE FOR SIGNIFICANT BACTERIURIA BY GRAM STAIN, ACRIDINE-ORANGE STAIN, AND THE AUTOBAC MTS SYSTEM
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DOI：
10.1016/0732-8893(84)90028-2
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发表时间：
1984-01-01
影响因子：
2.9
通讯作者：
TILTON, RC
中科院分区：
文献类型：
--
作者：
CROUT, FV;TILTON, RC
采用革兰氏染色、吖啶橙染色和Autobac MTS系统3种快速方法对送检的尿样进行细菌尿证据筛查。将筛选结果与琼脂平板半定量菌落计数结果进行比较。在这项对比研究中，检查了1055份尿样，其中146份(13.8%)有菌落计数。1.泰晤士报。3种尿液筛查方法检测该水平菌尿的敏感性均为98%，特异性分别为55.2%(吖啶橙)、66.0%(革兰氏染色)和83.2%(Autobac)。在检查的1055份尿样中，185份(17.5%)有菌落计数。1.泰晤士报。104/ml时，3种方法的敏感性为93%，特异性分别为56.7%(吖啶橙)、68.0%(革兰染色)和86.0%(Autobac)。对于任何级别的敏感性，Autobac尿液筛查被证明比革兰氏染色或吖啶橙方法更具特异性。吖啶橙染色是特异性最低的尿液筛查，尤其是在灵敏度上限时。
Urine specimens submitted for microbiologic examination were screened for evidence of bacteriuria by 3 rapid methods: Gram staining, acridine orange staining and the Autobac MTS system. The screening results were compared with those obtained by semiquantitative colony counts on agar plates. In this comparative study 1055 urine specimens were examined, of which 146 (13.8%) had colony counts of .gtoreq. 1 .times. 105/ml. All 3 urine screening methods detected this level of bacteriuria at a sensitivity of 98% and a specificity of 55.2% (acridine orange), 66.0% (Gram stain) and 83.2% (Autobac), respectively. Of the 1055 urine specimens examined, 185 (17.5%) had colony counts of .gtoreq. 1 .times. 104/ml, at which level the sensitivity of the 3 methods was 93% and the specificity was 56.7% (acridine orange), 68.0% (Gram stain) and 86.0% (Autobac), respectively. For any level of sensitivity, the Autobac urine screen was shown to be more specific than either the Gram stain or the acridine orange method. The acridine orange stain was the least specific urine screen, especially at the upper limits of sensitivity.