Div-Seq: Single-nucleus RNA-Seq reveals dynamics of rare adult newborn neurons.
Div-Seq: Single-nucleus RNA-Seq reveals dynamics of rare adult newborn neurons.
复制标题
DOI：
10.1126/science.aad7038
复制
发表时间：
2016-08-26
期刊：
影响因子：
--
通讯作者：
Regev A
中科院分区：
文献类型：
--
作者：
Habib N;Li Y;Heidenreich M;Swiech L;Avraham-Davidi I;Trombetta JJ;Hession C;Zhang F;Regev A
单细胞RNA测序提供了有关细胞类型和状态的丰富信息。然而，很难捕捉到罕见的动态过程，比如成年神经发生，因为从成年组织中分离罕见的神经元具有挑战性，而且每个阶段的标记物也很有限。在此，我们开发了Div - Seq技术，它将可扩展的单核RNA测序（sNuc - Seq）与通过EdU对增殖细胞进行脉冲标记相结合，以分析单个分裂细胞。sNuc - Seq和Div - Seq能够分别灵敏地识别密切相关的海马细胞类型以及追踪成年海马神经发生微环境中新生神经元的转录动态。我们还应用Div - Seq来识别和分析成年脊髓（一个非经典的神经发生区域）中罕见的新生γ - 氨基丁酸能神经元。sNuc - Seq和Div - Seq为对多种复杂组织进行无偏分析开辟了道路。
Single cell RNA-Seq provides rich information about cell types and states. However, it is difficult to capture rare dynamic processes, such as adult neurogenesis, because isolation of rare neurons from adult tissue is challenging and markers for each phase are limited. Here, we develop Div-Seq, which combines scalable single nucleus RNA-Seq (sNuc-Seq) with pulse labeling of proliferating cells by EdU to profile individual dividing cells. sNuc-Seq and Div-Seq can sensitively identify closely related hippocampal cell types and track transcriptional dynamics of newborn neurons within the adult hippocampal neurogenic niche, respectively. We also apply Div-Seq to identify and profile rare newborn GABAergic neurons in the adult spinal cord, a non-canonical neurogenic region. sNuc-Seq and Div-Seq open the way for unbiased analysis of diverse complex tissues.