Programmed cell death 5 correlates with disease activity and interleukin-17 in serum and synovial fluid of rheumatoid arthritis patients
Programmed cell death 5 correlates with disease activity and interleukin-17 in serum and synovial fluid of rheumatoid arthritis patients
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程序性细胞死亡 5 与类风湿关节炎患者血清和滑液中的疾病活动度和白细胞介素 17 相关
DOI：
10.3760/cma.j.issn.0366-6999.20122693
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发表时间：
2013-01-20
影响因子：
6.1
通讯作者：
Pan Huan
中科院分区：
文献类型：
--
作者：
Wang Jun-feng;Guan Zhen-peng;Pan Huan
背景 程序性细胞死亡5（PDCD5）是一种新型的凋亡调节基因，可促进多种肿瘤细胞的凋亡。研究表明，PDCD5 在体外可加速滑膜细胞的凋亡，这表明其在类风湿性关节炎 (RA) 发病机制中具有潜在作用。本研究检测RA患者血清和滑液中PDCD5的表达情况，及其对炎性细胞因子白细胞介素17（IL-17）表达的影响，并评估RA疾病活动度。方法采用酶联免疫吸附试验（ELISA）检测18例RA患者和22例骨关节炎（OA）患者血清和滑液中PDCD5和IL-17的水平。还检测了40名健康人的血清PDCD5浓度作为对照。作为疾病活动指标，还评估了C反应蛋白（CRP）、红细胞沉降率（ESR）、类风湿因子（RF）和X射线分级量表。结果RA患者血清和滑液PDCD5水平显着高于OA和健康对照。血清PDCD5水平与CRP和ESR呈负相关，且RF阴性组显着高于阳性组。 RA患者血清和滑液中PDCD5水平也与IL-17水平呈负相关。然而，不同Larsen分期的RA患者滑液PDCD5水平未检测到差异。结论 PDCD5影响RA发病机制。 RA中成纤维样滑膜细胞和炎症细胞凋亡不足可增加PDCD5蛋白的表达。由于PDCD5水平与疾病活动指数和IL-17水平呈负相关，PDCD5可能成为RA诊断和治疗的靶点。
Background Programmed cell death 5 (PDCD5) is a novel apoptotic regulatory gene that promotes apoptosis in various tumor cells. Studies have shown that PDCD5 accelerates the apoptosis of synoviocytes in vitro, implying a potential role in rheumatoid arthritis (RA) pathogenesis. This study examined the expression of PDCD5 in serum and synovial fluid of RA patients, its effect on the expression of inflammatory cytokine, interleukin-17 (IL-17), and the assessment of disease activity in RA.Methods PDCD5 and IL-17 levels in serum and synovial fluid from 18 patients with RA and 22 patients with osteoarthritis (OA) were detected using enzyme-linked immunosorbent assay (ELISA). Concentrations of serum PDCD5 in 40 healthy people were also detected as controls. As disease activity indices, C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), rheumatoid factor (RF), and X-ray grading scale were also evaluated.Results Serum and synovial fluid PDCD5 levels in RA patients were significantly higher than those in OA and healthy controls. Serum PDCD5 level was inversely correlated to CRP and ESR, and was significantly higher in the RF negative group than in the positive group. PDCD5 level was also negatively correlated with IL-17 levels both in serum and synovial fluid of RA patients. However, differences in synovial fluid PDCD5 level from RA patients at different Larsen stages were not detectable.Conclusions PDCD5 affects RA pathogenesis. Insufficient apoptosis of fibroblast-like synoviocytes and inflammatory cells in RA could increase the expression of PDCD5 protein. As PDCD5 levels correlated negatively with disease activity indices and IL-17 level, PDCD5 could become a target in the diagnosis and treatment of RA.