Distribution of Smoothened at hippocampal mossy fiber synapses
Distribution of Smoothened at hippocampal mossy fiber synapses
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DOI：
10.1097/wnr.0b013e32801421ce
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发表时间：
2007-03-05
期刊：
影响因子：
1.7
通讯作者：
Ruat, Martial
中科院分区：
文献类型：
--
作者：
Masdeu, Christelle;Bernard, Veronique;Ruat, Martial
七跨膜受体Smoothened是hedgehog信号转导所必需的。在成年期，Smoothened mRNA的最高密度出现在齿状回的颗粒细胞层。在那里，Smoothened的表达受到涉及突触能传递的突触活动的调节。然而，Smoothened蛋白的精确定位尚未报道。在这里，我们描述了Smoothened蛋白分布在海马苔藓纤维使用特定的Smoothened抗体。我们提供的证据，他们的突触前定位，并使用电子显微镜，表明Smoothened位于密切相关的突触囊泡，很少与质膜。这些研究结果表明，Smoothened是本地化突触前，并建议Smoothened信号转导可能涉及苔藓纤维功能的复杂方面。
The seven-transmembrane receptor Smoothened is essential for hedgehog signal transduction. In adulthood, the highest density of Smoothened mRNA is found in the granule cell layer of the dentate gyrus. There, Smoothened expression is regulated by the synaptic activity involving the glutamatergic transmission. The precise localization of Smoothened proteins, however, has not yet been reported. Here, we describe Smoothened protein distribution in the hippocampal mossy fibers using specific Smoothened antibodies. We provide evidences for their presynaptic localization, and using electron microscopy, show that Smoothened is located in close association with synaptic vesicles and rarely with the plasma membrane. These findings demonstrate that Smoothened is localized presynaptically and suggest that Smoothened signal transduction may be implicated in the complex aspects of mossy fiber function.