Phenotyping transgenic embryos: a rapid 3-D screening method based on episcopic fluorescence image capturing
Phenotyping transgenic embryos: a rapid 3-D screening method based on episcopic fluorescence image capturing
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DOI：
10.1038/ng785
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发表时间：
2002-01-01
期刊：
影响因子：
30.8
通讯作者：
Mohun, T
中科院分区：
文献类型：
--
作者：
Weninger, WJ;Mohun, T
我们描述了一种适用于小鼠胚胎常规三维(3-D)分析的技术，该技术基于连续切片蜡包埋标本时捕获的电子显微镜荧光图像。我们用这个程序描述了16三体(TS16)和Cited2缺失突变小鼠的心脏表型和相关血管，以及Myf5增强子/β-半乳糖苷酶转基因的表达模式。图像的一致性及其精确对齐非常适合使用视频动画、虚拟切除或商业3D重建软件包进行3D分析。荧光图像捕获技术为分析正常胚胎和转基因胚胎的胚胎和器官形态提供了一种简单而有力的工具。
We describe a technique suitable for routine three-dimensional (3-D) analysis of mouse embryos that is based on episcopic fluorescence images captured during serial sectioning of wax-embedded specimens. We have used this procedure to describe the cardiac phenotype and associated blood vessels of trisomic 16 (Ts16) and Cited2-null mutant mice, as well as the expression pattern of an Myf5 enhancer/beta-galactosidase transgene. The consistency of the images and their precise alignment are ideally suited for 3-D analysis using video animations, virtual resectioning or commercial 3-D reconstruction software packages. Episcopic fluorescence image capturing (EFIC) provides a simple and powerful tool for analyzing embryo and organ morphology in normal and transgenic embryos.