Effect of BioAggregate on osteoclast differentiation and inflammatory bone resorption in vivo
Effect of BioAggregate on osteoclast differentiation and inflammatory bone resorption in vivo
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BioAggregate 对体内破骨细胞分化和炎症骨吸收的影响
DOI：
10.1111/iej.12405
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发表时间：
2015-11-01
影响因子：
5
通讯作者：
Peng, B.
中科院分区：
文献类型：
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作者：
Zhang, J.;Zhu, L.;Peng, B.
目的研究BioAggregate(Innovative Bioceramix，Vancouver，BC，Canada)对脂多糖(LPS)诱导的体内骨破坏的影响，并与美国Tulsa，OK，Dentsply Tulsa Dental(MTA)进行比较。方法原代培养小鼠骨髓巨噬细胞，分别加入不同浓度的BioAggregate和MTA提取物。用细胞计数试剂盒8法检测细胞活力，用抗酒石酸酸性磷酸酶(TRAP)染色检测体外破骨细胞分化情况。建立脂多糖诱导的小鼠颅骨破坏模型，观察生物聚集物和MTA提取物的体内作用。第7天处死小鼠，取颅骨进行显微CT扫描、组织学分析和双重免疫荧光染色。结果生物聚集体提取物对骨髓基质细胞无明显的细胞毒性作用，对骨髓基质细胞有明显的抑制作用(P
AimTo investigate the effect of BioAggregate (Innovative Bioceramix, Vancouver, BC, Canada) on lipopolysaccharide (LPS)-induced bone destruction in vivo and to compare its performance with that of mineral trioxide aggregate (MTA; Dentsply Tulsa Dental, Tulsa, OK, USA).MethodologyMouse bone marrow macrophages (BMMs) were primary cultured and treated with several concentrations of BioAggregate and MTA extracts. Cell viability was measured with a Cell Counting Kit-8 assay, whilst in vitro osteoclast differentiation was evaluated with tartrate-resistant acid phosphatase (TRAP) staining. LPS-induced mouse calvarial bone destruction model was established to assess the effect of BioAggregate and MTA extracts in vivo. Mice were killed on day 7, and calvarial bones were prepared for microcomputed tomography scanning, histologic analysis and double-immunofluorescence staining. Statistical tests used were one-way anova followed by Student-Newman-Keuls test.ResultsBioAggregate extracts displayed no obvious cytotoxicity to BMMs and significantly inhibited (P