The non-haemadsorbing African swine fever virus isolate ASFV/NH/P68 provides a model for defining the protective anti-virus immune response
The non-haemadsorbing African swine fever virus isolate ASFV/NH/P68 provides a model for defining the protective anti-virus immune response
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DOI：
10.1099/0022-1317-82-3-513
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发表时间：
2001-03-01
影响因子：
3.8
通讯作者：
Martins, CLV
中科院分区：
文献类型：
--
作者：
Leitao, A;Cartaxeiro, C;Martins, CLV
非洲猪瘟病毒ASFV/NH/P68是一种自然发生的非血细胞吸附性非致死性分离株。对31头经口鼻或肌内接种该分离株的猪进行的纵向临床和免疫学研究定义了两组离散的动物：发展ASF慢性型病变的动物和保持无症状的动物。发生病变的动物在感染后晚期出现病毒血症和发热，NK活性水平接近对照动物，抗ASFV特异性抗体水平高，伴有明显的高丙种球蛋白血症，涉及IgG 1、IgG 2、IgM和伊加免疫球蛋白同种型。另一方面，感染后保持无症状的猪在感染后第14天后没有病毒血症或发热，并且具有升高的Nh细胞活性，但在整个实验期间血浆IG浓度正常，特异性抗病毒抗体浓度相对较低。重要的是，后一组猪病毒对高毒力ASFV/L 60分离株的后续挑战具有抵抗力，并且存活下来，上面检查和提到的任何参数都没有重大变化。最后，淋巴细胞增殖反应的有丝分裂原伴刀豆球蛋白A，植物血凝素和美洲商陆有丝分裂原没有压抑的两个临床定义组的猪。因此，进一步研究该感染模型可能为ASFV保护性免疫机制提供新的见解。
African swine fever virus ASFV/NH/P68 is a naturally occurring, non-haemadsorbing and non-fatal isolate. Longitudinal clinical and immunological studies on 31 pigs inoculated oronasally or intramuscularly with this isolate defined two discrete groups of animals: those developing ASF chronic type lesions and those remaining asymptomatic. Animals developing lesions had viraemia and fever late after infection, NK activity levels close to that of control animals and high levels of anti-ASFV specific antibodies together with a marked hypergammaglobulinaemia involving IgG1, lgG2, IgM and IgA immunoglobulin isotypes. Pigs remaining asymptomatic after infection, on the other hand, did not have viraemia or fever after day 14 post-infection and had elevated Nh cell activity, but normal plasma Ig concentrations and relatively low specific anti-virus antibody concentrations throughout the duration of the experiments. Importantly, the latter group of pigs virus were resistant to subsequent challenge with the highly virulent ASFV/L60 isolate and survived with no major changes in any of the parameters examined and referred to above. Finally, lymphoproliferative responses to the mitogens concanavalin A, phytohaemagglutinin and pokeweed mitogen were not depressed in either of the two clinically defined groups of pigs. Thus further studies with this infection model may provide new insights on mechanisms of protective immunity to ASFV.