A modified high-throughput analysis of PLFAs in soil.
A modified high-throughput analysis of PLFAs in soil.
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作者：
Ellis S;Ritz K
通过磷脂脂肪酸 (PLFA) 分析进行微生物群落分析是一项富有洞察力的技术，可阐明土壤中微生物组合的表型结构。先前的 PLFA 分析迭代使用了大量化学品，并且可能需要较长时间才能执行。实施该方法的另一个障碍是专用机械的成本和可用性。我们报告了一种高通量方法，该方法既缩短了从土壤中提取 PLFA 的时间，又减少了所需化学品的数量。
Microbial community profiling via phospholipid fatty-acid (PLFA) analysis is an insightful technique which elucidates the phenotypic structure of microbial assemblages within soil. Previous iterations of PLFA analysis have used large quantities of chemicals and can take extended periods of time to perform. Another barrier to the implementation of this method is the cost and availability of specialised machinery. We report on a high-throughput method which reduces both the time to extract PLFAs from soil and reduces the quantity of chemicals required.