A pyrene-degrading consortium from deep-sea sediment of the West Pacific and its key member Cycloclasticus sp. P1.
A pyrene-degrading consortium from deep-sea sediment of the West Pacific and its key member Cycloclasticus sp. P1.
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DOI：
10.1111/j.1462-2920.2008.01611.x
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发表时间：
2008-08
影响因子：
5.1
通讯作者：
Baojiang Wang;Qiliang Lai;Zhisong Cui;Tianfeng Tan;Z. Shao
中科院分区：
文献类型：
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作者：
Baojiang Wang;Qiliang Lai;Zhisong Cui;Tianfeng Tan;Z. Shao
从太平洋深海沉积物中获得了一个芘降解菌群。该菌能降解萘、菲、芘、苊、芴、蒽、荧蒽、2-甲基萘和2，6-二甲基萘等多环芳烃，但不能降解金、苯并[α]芘。采用平板培养和聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）方法，从该菌群中检测到22属72株细菌。在检测到的细菌中，以下属经常出现：黄杆菌属、破环菌属、新鞘氨醇菌属、盐单胞菌属、无色杆菌属、玫瑰果菌属和食烷菌属。前两个属在变性梯度凝胶电泳（DGGE）中显示出最强的条带，并出现在所有PAH处理。到目前为止，只有一个命名为P1的分离物被证实是芘降解菌。经鉴定，该菌为环裂蜱（Cycloclasticus），阳性率为100%。虽然P1可以独立降解芘，但其他细菌，如Novosphingobium sp.（Band 14），Halomonassp.（Band 16）和一种未知的细菌（Band 35），以某种方式参与了芘的降解;它们在稀释至灭绝的测试中坚持在该财团中，只要该财团被芘激发。然而，第二个最重要的成员黄杆菌属在高稀释度下从群落中逃逸。P1是该菌群中的重要成员，其细胞形态和碳源范围在该菌群中具有显著的优势。基于芘降解的中间体分析，推测P1可能走的是与以往报道不同的上途径。结合从P1中获得的与萘降解相关基因的同源性结果，推测P1降解芘可能采用了另一组目前检测到的独特基因。从太平洋沉积物中筛选到一株高效降解芘的菌群-这是第一份利用海洋环境中芘降解细菌多样性的报告。
A pyrene-degrading bacterial consortium was obtained from deep-sea sediments of the Pacific Ocean. The consortium degraded many kinds of polycyclic aromatic hydrocarbons (PAHs), including naphthalene, phenanthrene, pyrene, acenaphthene, fluorene, anthracene, fluoranthene, 2-methylnaphthalene and 2,6-dimethylnaphthalene, but it did not grow with chrysene and benzo[alpha]pyrene. With methods of plate cultivation and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), 72 bacteria belonging to 22 genera were detected from this consortium. Among the detected bacteria, the following genera frequently occurred: Flavobacterium, Cycloclasticus, Novosphingobium, Halomonas, Achromobacter, Roseovarius and Alcanivorax. The first two genera showed the strongest bands in denaturing gradient gel electrophoresis (DGGE) profiles and appeared in all PAH treatments. By now, only one isolate designated P1 was confirmed to be a pyrene degrader. It was identified to be Cycloclasticus spirillensus (100%). Although P1 can degrade pyrene independently, other bacteria, such as Novosphingobium sp. (Band 14), Halomonas sp. (Band 16) and an unidentified bacterium (Band 35), were involved in pyrene degradation in some way; they persist in the consortium in the test of dilution to extinction if only the consortium was motivated with pyrene. However, the secondary most important member Flavobacterium sp. evaded from the community at high dilutions. As a key member of the consortium, P1 distinguished itself by both cell morphology and carbon source range among the isolates of this genus. Based on intermediate analyses of pyrene degradation, P1 was supposed to take an upper pathway different from that previously reported. Together with the results of obtained genes from P1 homology with those responsible for naphthalene degradation, its degradation to pyrene is supposed to adopt another set of genes unique to presently detected. Summarily, an efficient pyrene-degrading consortium was obtained from the Pacific Ocean sediment, in which Cycloclasticus bacterium played a key role. This is the first report to exploit the diversity of pyrene-degrading bacteria in oceanic environments.