Distinguishing Signatures of Multipathway Conformational Transitions.
Distinguishing Signatures of Multipathway Conformational Transitions.
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DOI：
10.1103/physrevlett.118.088101
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发表时间：
2017-02-24
影响因子：
8.6
通讯作者：
Dudko OK
中科院分区：
文献类型：
--
作者：
Pierse CA;Dudko OK
生物分子折叠和结合成功能构象通常被认为是由多种途径而不是独特的途径介导的。然而，即使在人们可以“看到”个体构象转变的实验中，它们的随机性通常也会妨碍人们确定转变是通过一种还是多种途径发生的。我们在大分子的反应中建立了无模型的、可观察的特征，以强制明确地识别多个途径——即使途径本身无法被解析。统一的分析描述表明，通过多种途径，分子对外力的反应可以以多种方式形成，从而为单分子水平上的定制生物功能提供了丰富的设计空间。
The folding and binding of biomolecules into functional conformations are thought to be commonly mediated by multiple pathways rather than a unique route. Yet even in experiments where one can “see” individual conformational transitions, their stochastic nature generally precludes one from determining whether the transitions occurred through one or multiple pathways. We establish model-free, observable signatures in the response of macromolecules to force that unambiguously identify multiple pathways – even when the pathways themselves cannot be resolved. The unified analytical description reveals that, through multiple pathways, the response of molecules to external forces can be shaped in diverse ways, resulting in a rich design space for tailored biological function already at the single molecule level.