Proteins in action: femtosecond to millisecond structural dynamics of a photoactive flavoprotein.
Proteins in action: femtosecond to millisecond structural dynamics of a photoactive flavoprotein.
复制标题
DOI：
10.1021/ja407265p
复制
发表时间：
2013-10-30
影响因子：
15
通讯作者：
Meech SR
中科院分区：
文献类型：
--
作者：
Brust R;Lukacs A;Haigney A;Addison K;Gil A;Towrie M;Clark IP;Greetham GM;Tonge PJ;Meech SR
生命系统从根本上依赖于蛋白质对外界刺激的反应能力。这种反应的机制、潜在的结构动力学和调节的时间尺度是生物化学中的中心问题。在这里，我们探索了在几个光活性黄素蛋白中发现的BLUF结构域的结构动力学，它负责光激活功能，如趋光性和基因调节。使用瞬变振动光谱进行了长达10年的测量(从100飞秒到1毫秒)。发色团(黄素环)的局域动力学发生在皮秒到纳秒的时间尺度上，而随后的蛋白质结构重组在微秒内观察到。观察到与蛋白质不同振动相关的动力学的多个时间尺度，暗示了潜在的分层松弛路径。与更远的残基相比，直接与生色团氢键连接的残基的结构演变要慢得多。然而，抑制生物功能的点突变被证明是这种结构松弛途径的短路，它抑制了发生在离发色团更远的地方的变化，同时加速了靠近发色团的动态。
Living systems are fundamentally dependent on the ability of proteins to respond to external stimuli. The mechanism, the underlying structural dynamics, and the time scales for regulation of this response are central questions in biochemistry. Here we probe the structural dynamics of the BLUF domain found in several photoactive flavoproteins, which is responsible for light activated functions as diverse as phototaxis and gene regulation. Measurements have been made over 10 decades of time (from 100 fs to 1 ms) using transient vibrational spectroscopy. Chromophore (flavin ring) localized dynamics occur on the pico- to nanosecond time scale, while subsequent protein structural reorganization is observed over microseconds. Multiple time scales are observed for the dynamics associated with different vibrations of the protein, suggesting an underlying hierarchical relaxation pathway. Structural evolution in residues directly H-bonded to the chromophore takes place more slowly than changes in more remote residues. However, a point mutation which suppresses biological function is shown to ‘short circuit’ this structural relaxation pathway, suppressing the changes which occur further away from the chromophore while accelerating dynamics close to it.
登录
查看更多内容
影响因子：
2.9
作者：
Haigney, Allison;Lukacs, Andras;Tonge, Peter J.
通讯作者：
Tonge, Peter J.
DOI：
10.1073/pnas.1002951107
发表时间：
2010-04-20
影响因子：
11.1
作者：
Cho, Hyun Sun;Dashdorj, Naranbaatar;Anfinrud, Philip
通讯作者：
Anfinrud, Philip
DOI：
10.1073/pnas.0409035102
发表时间：
2005-05-17
影响因子：
11.1
作者：
Ihee, H;Rajagopal, S;Moffat, K
通讯作者：
Moffat, K
影响因子：
21.8
作者：
通讯作者：
--
影响因子：
2.9
作者：
Laan, W;Gauden, M;Hellingwerf, KJ
通讯作者：
Hellingwerf, KJ