Effects of OG-VI, a nucleoside-nucleotide mixture, on ischemic myocardial metabolism in dogs.
Effects of OG-VI, a nucleoside-nucleotide mixture, on ischemic myocardial metabolism in dogs.
复制标题
OG-VI（一种核苷-核苷酸混合物）对狗缺血性心肌代谢的影响。
DOI：
10.1097/00019501-199701000-00006
复制
发表时间：
1997
影响因子：
1.8
通讯作者：
K. Ichihara
中科院分区：
文献类型：
--
作者：
Y. Okazaki;S. Kano;S. Ogoshi;K. Ichihara
背景
OG-VI是一种由30 mmo1/L肌苷、30 mmo1/L 5‘-鸟苷钠、30 mmo1/L胞苷、22.5mmo1/L尿苷和7.5mmo1/L胸腺嘧啶核苷组成的溶液，已知可以改善心肌顿抑。
客观化
目的：研究OG-VI对缺血心肌能量和糖代谢的影响。
方法
戊巴比妥钠麻醉开胸犬冠状动脉左前降支结扎3min造成缺血。OG-VI生理盐水1.2 mmol/kg/min，或OG-VI 12 mmol/kg/min，以0.1ml/kg/min的速度注入OG-VI。缺血3min后取出心肌，用于测定组织能量和碳水化合物代谢产物水平。
结果
缺血明显降低ATP和1，6-二磷酸果糖含量，升高AMP、6-磷酸葡萄糖、6-磷酸果糖和乳酸含量。OG-VI灌流可显著保护缺血3min心肌组织腺嘌呤核苷酸水平，减轻缺血所致的糖代谢改变。
结论
OG-VI可改善缺血所致的心肌代谢紊乱。
BACKGROUND
OG-VI is a solution composed of 30 mmol/l inosine, 30 mmol/l sodium 5'-guanylate, 30 mmol/l cytidine, 22.5 mmol/l uridine, and 7.5 mmol/l thymidine, which is known to improve myocardial stunning.
OBJECTIVE
To study the effect of OG-VI on myocardial energy and carbohydrate metabolism during ischemia.
METHODS
Ischemia was induced in pentobarbital-anesthetized open-chested dogs for 3 min by left anterior descending coronary artery ligation. Saline, 1.2 mmol/kg per min OG-VI, or 12 mmol/kg per min OG-VI was infused at 0.1 ml/kg per min, starting 30 min before the onset of ischemia. The myocardium was extracted 3 min after the onset of ischemia, and used to determine the tissue levels of energy and carbohydrate metabolites.
RESULTS
Ischemia decreased the levels of ATP and fructose 1,6 diphosphate significantly, and increased those of AMP, glucose 6 phosphate, fructose 6 phosphate and lactate. OG-VI infusion preserved the adenine nucleotide levels significantly in 3 min ischemic hearts, and attenuated the ischemia-induced alteration of carbohydrate metabolism.
CONCLUSION
OG-VI may improve the myocardial metabolic derangement induced by ischemia.