Shope fibroma virus. III: Stimulation of the growth of the noncytocidal strain by a tumor promoter.
Shope fibroma virus. III: Stimulation of the growth of the noncytocidal strain by a tumor promoter.
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肖普纤维瘤病毒。
DOI：
10.1111/j.2164-0947.1983.tb02797.x
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Pogo,BG
中科院分区：
文献类型：
--
作者：
Pogo,BG
材料和方法兔角膜成纤维细胞（SIRC）从美国类型培养收集（Rockville， MD）中获得，在最小基本培养基（Auto-Pow MEM; Flow Laboratories, Rockville， MD）和10%胎牛血清中培养成单层，并在37℃的空气和二氧化碳混合气体中保持。两株Shope纤维瘤病毒在SIRC细胞单层中繁殖。通过琼脂下SIRC单层细胞中斑块的产生来检测SFV-I的感染性，而通过计算病灶的数量来检测SFV-W的感染性
MATERIALS AND METHODSRabbit cornea fibroblast cells (SIRC) were obtained from the American Type Culture Collection (Rockville, MD) and cultured as monolayers in minimal essential medium (Auto-Pow MEM; Flow Laboratories, Rockville, MD) and 10% fetal calf serum and maintained at 37OC in a gas mixture of air and C02. The two strains of Shope fibroma virus were propagated in SIRC cell monolayers. Infectivity of SFV-I was measured by the production of plaques in SIRC cell monolayers under agar, while SFV-W was measured by counting the number of foci