A novel virus-host cell membrane interaction. Membrane voltage-dependent endocytic-like entry of bacteriophage straight phi6 nucleocapsid.
A novel virus-host cell membrane interaction. Membrane voltage-dependent endocytic-like entry of bacteriophage straight phi6 nucleocapsid.
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一种新型的病毒宿主细胞膜相互作用。噬菌体的膜电压依赖性内吞样的进入直phi6核素。
DOI：
10.1083/jcb.147.3.671
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发表时间：
1999-11-01
影响因子：
7.8
通讯作者：
Bamford, D H
中科院分区：
文献类型：
--
作者：
Poranen, M M;Daugelavicius, R;Ojala, P M;Hess, M W;Bamford, D H
病毒与细胞相互作用的研究已被证明在阐明重要的细胞过程中是有价值的。有趣的是，在真核系统中发现的某些病毒-宿主膜相互作用似乎也在原核生物中起作用（Bamford，D.H.，M. Romantschuk和P. J. Somerharju，1987年。EMBO（Eur.摩尔Biol. Organ.）J. 6：1467-1473; Romantschuk，M.，V.M. Olkkonen和D.H.班福德1988. EMBO（Eur.摩尔Biol. Organ.）J. 7：1821-1829）。φ6是一种感染革兰氏阴性细菌的包膜双链RNA病毒。通过病毒膜和宿主外膜之间的融合启动病毒进入，随后通过内吞样途径将病毒核衣壳（覆盖有蛋白质壳的RNA聚合酶复合物）递送到宿主胞质溶胶中。在这项研究中，我们分析了核衣壳与宿主质膜的相互作用，并展示了一种新的方法来解剖核衣壳进入过程的早期事件。核衣壳与质膜的初始结合不依赖于膜电压（Δ V）和K+和H+梯度。然而，随后的内化依赖于质膜电压（Δ V），但不需要高ATP水平或K+和H+梯度。此外，核衣壳壳蛋白P8是介导膜-核衣壳相互作用的病毒组分。
Studies on the virus–cell interactions have proven valuable in elucidating vital cellular processes. Interestingly, certain virus–host membrane interactions found in eukaryotic systems seem also to operate in prokaryotes (Bamford, D.H., M. Romantschuk, and P.J. Somerharju, 1987. EMBO (Eur. Mol. Biol. Organ.) J. 6:1467–1473; Romantschuk, M., V.M. Olkkonen, and D.H. Bamford. 1988. EMBO (Eur. Mol. Biol. Organ.) J. 7:1821–1829). φ6 is an enveloped double-stranded RNA virus infecting a gram-negative bacterium. The viral entry is initiated by fusion between the virus membrane and host outer membrane, followed by delivery of the viral nucleocapsid (RNA polymerase complex covered with a protein shell) into the host cytosol via an endocytic-like route. In this study, we analyze the interaction of the nucleocapsid with the host plasma membrane and demonstrate a novel approach for dissecting the early events of the nucleocapsid entry process. The initial binding of the nucleocapsid to the plasma membrane is independent of membrane voltage (ΔΨ) and the K+ and H+ gradients. However, the following internalization is dependent on plasma membrane voltage (ΔΨ), but does not require a high ATP level or K+ and H+ gradients. Moreover, the nucleocapsid shell protein, P8, is the viral component mediating the membrane–nucleocapsid interaction.