The distribution of granulocyte-macrophage colony-stimulating factor and its receptor in the developing human fetus
The distribution of granulocyte-macrophage colony-stimulating factor and its receptor in the developing human fetus
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DOI：
10.1203/00006450-199910000-00002
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发表时间：
1999-10-01
影响因子：
3.6
通讯作者：
Juul, SE
中科院分区：
文献类型：
--
作者：
Dame, JB;Christensen, RD;Juul, SE
本研究旨在探讨粒细胞-巨噬细胞集落刺激因子（GM-CSF）及其受体（GM-CSF-R）在人胎儿中的分布。采用逆转录聚合酶链反应（RT-PCR）检测人胚胎器官中GM-CSF和GM-CSF-R mRNA的表达，免疫组织化学方法检测组织中细胞特异性蛋白的表达。还通过ELISA在脊髓液和血浆的配对样品中测量GM-CSF。在肺巨噬细胞、脾、肾上腺皮质、胎盘和CNS（包括神经元和星形胶质细胞）中检测到GM-CSF mRNA和/或蛋白。在39份脑脊液标本中，有10份用ELISA法检测到GM-CSF。GM-CSF-R mRNA在所有受试器官中均有表达。GM-CSF-R在大多数器官中仅限于巨噬细胞，但脑、神经元和胶质细胞呈免疫反应性。我们的结论是，GM-CSF产生于肺，脾，肾上腺，胎盘和神经组织在人类胎儿发育和GM-CSF反应细胞包括巨噬细胞，神经元和神经胶质细胞。
The purpose of the present study was to determine the distribution of granulocyte-macrophage colony-stimulating factor (GM-CSF) and its receptor (GM-CSF-R) in the human fetus. We used reverse transcription PCR to detect GM-CSF and GM-CSF-R mRNA in human fetal organs at 8 and 16 wk postconception, and cell-specific protein expression was localized in tissues by immunohistochemistry. GM-CSF was also measured by ELISA in paired samples of spinal fluid and plasma. GM-CSF mRNA and/or protein were detected in lung macrophages, spleen, adrenal cortex, placenta, and CNS including neurons and astrocytes. GM-CSF was detected by ELISA in 10 of the 39 cerebrospinal fluid samples tested. GM-CSF-R mRNA expression was present in all organs tested. Immunoreactivity for GM-CSF-R in most organs was limited to macrophages, but, brain, neurons and glial cells showed immunoreactivity. We conclude that GM-CSF is produced in lung, spleen, adrenal, placenta, and neural tissues during human fetal development and that GM-CSF-responsive cells include macrophages, neurons, and glial cells.