Differential expression of miRNA patterns in renal cell carcinoma and nontumorous tissues
Differential expression of miRNA patterns in renal cell carcinoma and nontumorous tissues
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DOI：
10.1007/s00432-009-0726-x
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发表时间：
2010-06-01
影响因子：
3.6
通讯作者：
Ma, Chuanxiang
中科院分区：
文献类型：
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作者：
Yi, Zhengjun;Fu, Yurong;Ma, Chuanxiang
miRNAs是短的单链RNA，在转录和翻译水平上与基因调控相关。在多种人类癌症中发现了它们表达的变化。本研究旨在探讨肾癌透明细胞亚型组织中miRNA的表达谱，进一步了解肾癌发生的分子机制。我们分析了30对肾细胞癌及其癌旁组织中miRNA的表达谱，使用含有完整的人类成熟和前体miRNA序列的哺乳动物miRNA微阵列。实时荧光定量PCR检测芯片结果，发现86个miRNA在肾癌组织中有差异表达，其中38个miRNA在肾癌组织中的表达高于正常对照组，而48个miRNA在肾细胞癌中的表达低于NT。定量真实的-时间PCR分析证实了微阵列数据。该报告支持许多miRNA在肾癌中表达发生改变，其表达谱可能为病理生理学研究提供有用的线索。然而，miRNA与肾癌的关系及其在肾癌发生中的作用还需要进一步的长期研究。
MiRNAs are short single stranded RNAs that are associated with gene regulation at the transcriptional and translational level. Changes in their expression were found in a variety of human cancers. In the present study, we aimed to investigate expression profiles of miRNA (miRNA) in the clear cell subtype of kidney cancer and to develop further understanding of the molecular mechanisms involved in the pathogenesis of renal cell carcinoma.We analyzed the miRNA expression profiles in 30 pairs of renal cell carcinoma and adjacent nontumorous tissue (NT), using a mammalian miRNA microarray containing whole human mature and precursor miRNA sequences. Real-time quantitative PCR was performed to confirm the array results.The miRNA microarray chip analysis identified 86 miRNAs differentially expressed in renal cell carcinoma tissues and a total of 38 miRNAs exhibited higher expression in the renal cell carcinoma samples than that in the NT samples, while 48 miRNA demonstrated lower expression in the renal cell carcinoma samples than that in the NT samples.Quantitative real-time PCR analysis confirmed microarray data. The report supports that many miRNA expressions were altered in renal carcinoma, whose expression profiling may provide a useful clue for the pathophysiology research. However, further longer-term researches are required to investigate the relationship between miRNA and renal carcinoma as well as their role in carcinogenesis.