Hybrid nanogels with physical and chemical cross-linking structures as nanocarriers.
Hybrid nanogels with physical and chemical cross-linking structures as nanocarriers.
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DOI：
10.1002/mabi.200500051
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发表时间：
2005-08
影响因子：
4.6
通讯作者：
N. Morimoto;T. Endo;M. Ohtomi;Y. Iwasaki;K. Akiyoshi
中科院分区：
文献类型：
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作者：
N. Morimoto;T. Endo;M. Ohtomi;Y. Iwasaki;K. Akiyoshi
通过胆固醇基普鲁兰多糖（CHP）与甲基丙烯酰基（CHPMA）的自组装制备了可聚合纳米凝胶。以2-甲基丙烯酰氧乙基磷酰胆碱（MPC）为引发剂，在稀水溶液中进行自由基聚合。用TEM、SEC-MALS和荧光猝灭技术研究了聚合物在水中的溶液性质。通过使用CHPMA纳米凝胶作为种子纳米凝胶，获得了单分散的CHPMA-MPC杂化纳米凝胶（CM纳米凝胶）（回转半径为25-30 nm）。CM纳米凝胶具有与胆固醇基物理交联和与MPC聚合物链化学交联的双重交联结构。CM纳米凝胶捕获热变性的碳酸酐酶B（CA B）并防止其聚集。胆固醇基和环糊精的主客体相互作用使纳米凝胶保持了对酶的捕获和释放能力。
Polymerizable nanogels were prepared by self-assembly of cholesteryl group-bearing pullulan (CHP) with methacryloyl groups (CHPMA). The CHPMA nanogel was polymerized with 2-methacryloyloxyethyl phosphorylcholine (MPC) by radical polymerization in dilute aqueous solution. The solution properties of the polymers in water were investigated by TEM, SEC-MALS, and fluorescence quenching technique. Monodispersed hybrid nanogels of CHPMA-MPC (CM nanogels) (25-30 nm in radius of gyration) were obtained by using CHPMA nanogel as a seed-nanogel. CM nanogels have a dual cross-linking structure that is physically cross-linked with the cholesteryl groups and chemically cross-linked with the MPC polymer chains. CM nanogels trap heat-denatured carbonic anhydrase B (CAB) and prevent their aggregations. The nanogels maintained the ability of trapping and releasing enzymes by host-guest interaction of cholesteryl group and cyclodextrin.