Promotion effect of alginate on chitinase production by Wasabia japonica
Promotion effect of alginate on chitinase production by Wasabia japonica
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海藻酸盐对山葵产几丁质酶的促进作用
DOI：
10.1007/bf00168473
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发表时间：
1996
影响因子：
--
通讯作者：
Hideo Tanaka
中科院分区：
文献类型：
--
作者：
H. Aoyagi;M. Okada;Chiharu Akimoto;Hiroichi Katsuyama;S. Yoshida;I. Kusakabe;Hideo Tanaka
当海藻酸盐（1.0 g/l）添加到山葵细胞培养物中时，细胞生长受到轻微抑制（11-17%），但几丁质酶产量和特异性几丁质酶生产率均增加。当向培养物中添加众所周知的引发剂壳聚糖（1.0 g/l）时，也观察到类似的结果。这些结果表明藻酸盐充当一种引发剂。当使用低分子量海藻酸盐（低聚物）时，海藻酸盐对几丁质酶产生的促进作用更加显着。与游离细胞相比，向W. japonica原生质体培养物中添加海藻酸盐导致几丁质酶生产力增加3倍。
When alginate (1.0 g/l) was added to Wasabia japonica cell culture, cell growth was slightly inhibited (11–17%) but both the chitinase production and the specific chitinase productivity increased. Similar results were also observed when chitosan (1.0 g/l), which is well known as an elicitor, was added to the culture. These results suggest that alginate act as a kind of elicitor. Promotion effect of alginate on chitinase production was more remarkable when low molecular weight alginate (oligomer) was used. In comparison with free cells, addition of alginate to W. japonica protoplast culture resulted to 3 times increase in the chitinase productivity.