Polyphenolic profiles in eight apple cultivars using high-performance liquid chromatography (HPLC)
Polyphenolic profiles in eight apple cultivars using high-performance liquid chromatography (HPLC)
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DOI：
10.1021/jf0346298
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发表时间：
2003-10-08
影响因子：
6.1
通讯作者：
Zhu, HH
中科院分区：
文献类型：
--
作者：
Tsao, R;Yang, R;Zhu, HH
苹果多酚类化合物可能具有与人体健康相关的重要生理功能。不同的多酚可能具有不同的生物活性，包括抗氧化活性。研究了不同苹果品种和苹果不同部位多酚类化合物的含量。安大略地区8个苹果品种的总酚和单酚含量差异显著，果皮中的含量高于果肉。在供试品种中，Red Delicious和北方Spy的浓度最高，Empire最低。五个主要的多酚类化合物，共16个确定的个人化合物被发现，其中的二羟基肉桂酸酯，根皮苷，黄烷-3-醇被发现在果肉和果皮，而槲皮素苷几乎完全被发现在果皮。矢车菊素3-半乳糖苷是红苹果果皮所特有的，仅在红苹果果皮中发现。在苹果果皮和果肉中，多酚类物质的主要成分是原花青素，其次是果皮中的槲皮素苷和果肉中的羟基肉桂酸酯。3-羟基根皮素2 '-木糖葡萄糖苷是从苹果中新鉴定的一种植物。本研究结果将有助于进一步了解苹果多酚类物质的组成及其生理保健功能。
Polyphenolic compounds of apple may play an important role in physiologic functions related to human health. Different polyphenolics may have varied biological activities including antioxidant activity. The objective of this study was to investigate the profiles of polyphenolic compounds in different apple varieties and different parts of an apple. The total and individual polyphenolics differed significantly among the eight apple cultivars grown in Ontario, and the peels had higher concentrations than the fleshes. Among the tested cultivars, Red Delicious and Northern Spy had the highest concentrations and Empire the lowest. Five major polyphenolic groups with a total of 16 identified individual compounds were found, among which the dihydroxycinnamic acid esters, phloretin glycosides, and flavan-3-ols were found in both flesh and peel, whereas quercetin glycosides were almost exclusively found in the peel. Cyanidin 3-galactoside was unique to and found only in red apple peels. In both apple peel and flesh, the predominant group of polyphenolics was the procyanidins, followed by quercetin glycosides in the peel and hydroxycinnamic acid esters in the flesh. 3-Hydroxyphloretin 2'-xyloglucoside was newly identified in apple. The results obtained in this study will further the understanding of the polyphenolic composition of apples and their roles in health-promoting physiological functions.