Carriage of respiratory tract pathogens and molecular epidemiology of Streptococcus pneumoniae colonization in healthy children attending day care centers in Lisbon, Portugal
Carriage of respiratory tract pathogens and molecular epidemiology of Streptococcus pneumoniae colonization in healthy children attending day care centers in Lisbon, Portugal
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DOI：
10.1089/mdr.1999.5.19
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发表时间：
1999-03-01
影响因子：
2.6
通讯作者：
Tomasz, A
中科院分区：
文献类型：
--
作者：
de Lencastre, H;Kristinsson, KG;Tomasz, A
为了确定葡萄牙城市日托中心（DCC）儿童中抗生素耐药呼吸道病原体的携带率，选择了里斯本的7个DCC，以确定6个月至6岁儿童的肺炎链球菌、流感嗜血杆菌和卡他莫拉菌鼻咽定植率，在1996年1月至3月期间研究的586名儿童中，47%携带肺炎链球菌，72%携带流感嗜血杆菌，54%携带卡他莫拉菌。24%的肺炎球菌对青霉素的敏感性降低，其中大多数属于血清群19、23、14和6，另外19%对青霉素完全敏感，但对其他抗菌剂的敏感性降低，这些分离株表达血清群6、11、14、18、19和34。大多数（96%）卡他莫拉菌和20%流感嗜血杆菌由于产生β-内酰胺酶而对青霉素耐药，近期抗菌药物的使用与青霉素非敏感性肺炎球菌和产β-内酰胺酶流感嗜血杆菌的携带有关（p < 0.05），个体DCC在它们的抗生素耐药性流感嗜血杆菌和肺炎链球菌的携带率方面彼此显著不同，抗生素耐药S.通过分子指纹技术对肺炎克雷伯氏菌分离株进行的鉴定表明，每种DCC都具有独特的微生物谱，表明它们之间几乎没有（如果有的话）耐药微生物植物群的交换。一个例外是存在属于两个国际传播的流行性克隆的分离株：表达血清型23 F的多重耐药西班牙/美国克隆，以及青霉素和磺胺甲恶唑-甲氧苄啶耐药法国/西班牙克隆（血清型14），分别在四个和三个DCC中检测到。
In an effort to establish the rate of carriage of antibiotic resistant respiratory pathogens in children attending urban day care centers (DCC) in Portugal, seven DCC in Lisbon were selected for determining the rate of nasopharyngeal colonization of children between the ages of 6 months to 6 years by Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis, Of the 586 children studied between January and March 1996, 47% carried S, pneumoniae, 72% H, influenzae, and 54% M, catarrhalis, Twenty-four percent of the pneumococci had reduced susceptibility to penicillin, and most of these belonged to serogroups 19, 23, 14, and 6, An additional 19% were fully susceptible to penicillin but showed decreased susceptibility to other antimicrobials, These isolates expressed serogroups 6, 11, 14, 18, 19, and 34, The majority (96%) of M, catarrhalis and 20% of H, influenzae were penicillin resistant due to the production of beta-lactamases, Recent antimicrobial use was associated with carriage of penicillin non-susceptible pneumococci and beta-lactamase producing H, influenzae (p < 0.05), Individual DCC differed substantially from one another in their rates of carriage of antibiotic resistant H, influenzae and S, pneumoniae, Characterization of antibiotic resistant S. pneumoniae isolates by molecular fingerprinting techniques showed that each DCC had a unique microbiological profile, suggesting little, if any, exchange of the resistant microbial flora among them. An exception to this was the presence of isolates belonging to two internationally spread epidemic clones: the multiresistant Spanish/USA clone expressing serotype 23F, and the penicillin and sulfamethoxazole-trimethoprim resistant French/Spanish clone (serotype 14) which were detected in four and three DCC, respectively.