Isolation of proteins associated with the DNA-bound estrogen receptor alpha.
Isolation of proteins associated with the DNA-bound estrogen receptor alpha.
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DOI：
10.1007/978-1-60327-378-7_13
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
Nardulli, Ann M
中科院分区：
文献类型：
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作者：
Schultz-Norton, Jennifer R;Ziegler, Yvonne S;Likhite, Varsha S;Nardulli, Ann M
调控基因表达是一个复杂的过程，需要多个转录因子与其同源识别序列相互作用。虽然这些dna结合的转录因子是基因表达的主要驱动因素，但转录因子改变基因表达的能力受到其与宿主协同调节蛋白的关联的影响，这些蛋白被招募到dna结合的转录因子中。我们已经开发了一种新的方法来分离与dna结合的雌激素受体α (ERα)相关的大的蛋白质复合物，使用琼脂糖为基础的电泳迁移转移试验（EMSA）。这种方法应该很容易适应于各种培养细胞系、DNA序列和转录因子，并有可能提供有关影响基因表达的各种调节蛋白的有价值信息。
Regulating gene expression is a complex process requiring the interaction of multiple transcription factors with their cognate recognition sequences. While these DNA-bound transcription factors are the primary drivers of gene expression, the capacity of a transcription factor to alter gene expression is tempered by its association with a host of coregulatory proteins that are recruited to the DNA-bound transcription factor. We have developed a novel approach to isolate large complexes of proteins associated with the DNA-bound estrogen receptor α (ERα) using an agarose-based electrophoretic mobility shift assay (EMSA). This method should be readily adapted to a variety of cultured cell lines, DNA sequences, and transcription factors and has the potential to provide valuable information about a wide variety of regulatory proteins involved in influencing gene expression.