Cellular and antibody responses directed against the HIV-1 principal neutralizing domain in HIV-1-infected children.
Cellular and antibody responses directed against the HIV-1 principal neutralizing domain in HIV-1-infected children.
复制标题
针对 HIV-1 感染儿童中 HIV-1 主要中和结构域的细胞和抗体反应。
DOI：
10.1089/aid.1991.7.839
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发表时间：
1991
影响因子：
1.5
通讯作者：
Goldstein,H
中科院分区：
文献类型：
--
作者：
Wiseman,G;Rubinstein,A;Martinez,P;Lambert,S;Devash,Y;Goldstein,H
抗体应答的主要中和结构域（PND）位于gp 120的V3可变区内，也可以刺激T细胞应答。在一些感染人类免疫缺陷病毒（HIV）的成人中，HIV-1特异性T细胞应答可以通过证明体外增殖为HIV-1蛋白和肽来检测。在其他HIV-1感染的成人中，HIV-1特异性T细胞应答可能涉及在T细胞增殖不存在的情况下分泌白细胞介素2（IL-2）。为了阐明儿童对PND的T细胞反应，我们检测了19名HIV-1感染儿童外周血淋巴细胞对含有HIVMN主要中和结构域的高度保守序列的肽（PND-MN）的增殖和IL-2分泌反应。PND-MN刺激诱导2名儿童的淋巴细胞增殖和5名儿童的淋巴细胞分泌IL-2。在一个3个月大的婴儿，在体外细胞反应的PND-MN表明HIV-1感染之前，在她的血清中检测p24抗原。尽管在所有检查的儿童中检测到针对PND-MN的抗体，但对PND-MN的高亲和力/亲合力抗体的存在与对PND-MN的细胞应答的存在相关。因此，在HIV-1感染的儿童中，在不存在增殖反应的情况下，HIV-1特异性T细胞反应可以通过测定IL-2分泌反应来评估，并且与高亲和力/亲合力抗体的产生相关。
The principal neutralizing domain (PND) for antibody response is located within the V3 variable region of gp 120 and can also stimulate T-cell responses. In some adults infected with human immunodeficiency virus (HIV) an HIV-1-specific T-cell response can be detected by demonstrating in vitro proliferation to HIV-1 proteins and peptides. In other HIV-1 infected adults an HIV-1-specific T-cell response can involve interleukin 2 (IL-2) secretion in the absence of T-cell proliferation. To elucidate the T-cell responses to PND in children, we examined the proliferative and the IL-2 secretory responses of peripheral blood lymphocytes from 19 HIV-1-infected children toward a peptide which contained a highly conserved sequence of the principal neutralizing domain of HIVMN(PND-MN). Stimulation with PND-MN induced proliferation of lymphocytes from 2 of the children and IL-2 secretion by lymphocytes from 5 of the children. In a 3-month-old infant, the in vitro cellular response to the PND-MN indicated HIV-1 infection prior to the detection p24 antigen in her serum. Although antibodies directed against PND-MN were detected in all but one of the children examined, the presence of high-affinity/avidity antibodies to the PND-MN correlated with the presence of a cellular response to PND-MN. Thus, in HIV-1-infected children an HIV-1 specific T-cell response in the absence of a proliferative response can be assessed by determination of the IL-2 secretory response and correlates with the generation of high-affinity/avidity antibodies.