Functionalization density dependence of single-walled carbon nanotubes cytotoxicity in vitro
Functionalization density dependence of single-walled carbon nanotubes cytotoxicity in vitro
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DOI：
10.1016/j.toxlet.2005.08.011
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发表时间：
2006-02-20
影响因子：
3.5
通讯作者：
Colvin, VL
中科院分区：
文献类型：
--
作者：
Sayes, CM;Liang, F;Colvin, VL
细胞的细胞毒性反应取决于单壁碳纳米管（SWNT）的功能化程度。在表征了一组水分散性单壁碳纳米管后，我们对培养的人皮肤成纤维细胞（HDF）进行了体外细胞毒性筛选。在该暴露中使用的SWNT样品包括SWNT-苯基-SO 3 H和SWNT-苯基-SO 3 M（碳/-苯基-SO 3X比率为18、41和80的六个样品）、SWNT-苯基-（COOH）（2）（碳/-苯基-（COOH）（2）比率为23的一个样品）和在1%Pluronic F108中稳定的未衍生化的SWNT。我们已经发现，随着侧壁官能化程度的增加，单壁碳纳米管样品的细胞毒性变小。此外，侧壁官能化的SWNT样品的细胞毒性比表面活性剂稳定的SWNT低得多。即使细胞死亡不超过50%的细胞与侧壁官能化的SWNT，光学和原子力显微镜显示细胞膜和水分散性SWNT之间的直接接触，即在水悬浮液中的SWNT沉淀出来，并选择性地存款在膜上。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
The cytotoxic response of cells in culture is dependant on the degree of functionalization of the single-walled carbon nanotube (SWNT). After characterizing a set of water-dispersible SWNTs, we performed in vitro cytotoxicity screens on cultured human dermal fibroblasts (HDF). The SWNT samples used in this exposure include SWNT-phenyl-SO3H and SWNT-phenyl-SO3M (six samples with carbon/-phenyl-SO3X ratios of 18, 41, and 80), SWNT-phenyl-(COOH)(2) (one sample with carbon/-phenyl-(COOH)(2) ratio of 23), and underivatized SWNT stabilized in 1% Pluronic F108. We have found that as the degree of sidewall functionalization increases, the SWNT sample becomes less cytotoxic. Further, sidewall functionalized SWNT samples are substantially less cytotoxic than surfactant stabilized SWNTs. Even though cell death did not exceed 50% for cells dosed with sidewall functionalized SWNTs, optical and atomic force microscopies show direct contact between cellular membranes and water-dispersible SWNTs; i.e. the SWNTs in aqueous suspension precipitate out and selectively deposit on the membrane. (c) 2005 Elsevier Ireland Ltd. All rights reserved.