Proteomic profiling of Rhipicephalus (Boophilus) microplus midgut responses to infection with Babesia bovis
Proteomic profiling of Rhipicephalus (Boophilus) microplus midgut responses to infection with Babesia bovis
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DOI：
10.1016/j.vetpar.2007.12.027
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发表时间：
2008-04-15
影响因子：
2.6
通讯作者：
Scoles, Glen A.
中科院分区：
文献类型：
--
作者：
Rachinsky, Anna;Guerrero, Felix D.;Scoles, Glen A.
研究了未感染巴贝斯虫和感染巴贝斯虫的南方牛蜱中肠组织中蛋白表达的差异，建立了包含成功传播巴贝斯虫相关蛋白的蛋白质组数据库。液相等电聚焦结合一维或二维凝胶电泳，大大提高了中肠膜蛋白的电泳分离效果。选择差异表达蛋白进行毛细管-高效液相色谱-电喷雾串联质谱（HPLC-ESI-MS/MS）分析。在鉴定的受巴贝虫感染的蜱虫中肠蛋白中，有6种蛋白与信号传导过程有关，包括3种Ca2+结合蛋白、1种鸟嘌呤核苷酸结合蛋白、1种具有信号肽活性的蛋白和1种转座相关受体蛋白。5种代谢酶的上调表明寄生虫诱导了电子和质子传递、蛋白质加工和维甲酸代谢的变化。下调的蛋白包括分子伴侣蛋白、细胞骨架蛋白和禁止蛋白家族的多功能蛋白。这些蛋白的鉴定可能为了解牛b及其蜱虫载体之间的分子相互作用提供新的见解，并可能导致鉴定抗蜱虫和传播阻断候选疫苗。Elsevier B.V.出版
Differences in protein expression in midgut tissue of uninfected and Babesia bovis-infected southern cattle ticks, Rhipicephalus (Boophilus) microplus, were investigated in an effort to establish a proteome database containing proteins involved in successful pathogen transmission. The electrophoretic separation of midgut membrane proteins was greatly improved by using liquid-phase isoelectric focusing combined with one-dimensional or two-dimensional (2-D) gel electrophoresis. A selection of differentially expressed proteins were subjected to analysis by capillary-HPLC-electrospray tandem mass spectrometry (HPLC-ESI-MS/MS). Among the identified Babesia-affected tick midgut proteins were six proteins that are implicated in signaling processes, including three Ca2+-binding proteins, a guanine nucleotide-binding protein, a protein with signal peptide activity and a translocon-associated receptor protein. Up-regulation of five metabolic enzymes indicated parasite-induced changes in electron and proton transport, protein processing and retinoic acid metabolism. Among the down-regulated proteins were a molecular chaperone, a cytoskeletal protein and a multifunctional protein of the prohibitin family. Identification of these proteins may provide new insights into the molecular interactions between B. bovis and its tick vector, and could lead to identification of anti-tick and transmission-blocking vaccine candidates. Published by Elsevier B.V.