TRANSFER OF THE TOXIN PROTEIN GENES OF BACILLUS-SPHAERICUS INTO BACILLUS-THURINGIENSIS SUBSP ISRAELENSIS AND THEIR EXPRESSION
TRANSFER OF THE TOXIN PROTEIN GENES OF BACILLUS-SPHAERICUS INTO BACILLUS-THURINGIENSIS SUBSP ISRAELENSIS AND THEIR EXPRESSION
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DOI：
10.1128/aem.56.2.340-344.1990
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发表时间：
1990-02-01
影响因子：
4.4
通讯作者：
SZULMAJSTER, J
中科院分区：
文献类型：
--
作者：
BOURGOUIN, C;DELECLUSE, A;SZULMAJSTER, J
编码球形芽孢杆菌毒素决定簇的基因已在苏云金芽孢杆菌亚种的无毒株和有毒株中表达。以色列人。在这两种情况下，球形芽孢杆菌毒素蛋白在苏云金芽孢杆菌的产孢期都有较高水平的产生，并以结晶结构的形式积累。表达球形芽孢杆菌和苏云金芽孢杆菌亚种的苏云金杆菌转化子。白纹伊蚊毒素对埃及伊蚊、斯氏按蚊和淡色库蚊幼虫的毒性没有明显增强。
The genes encoding the toxic determinants of Bacillus sphaericus have been expressed in a nontoxic and a toxic strain of Bacillus thuringiensis subsp. israelensis. In both cases, the B. sphaericus toxin proteins were produced at a high level during sporulation of B. thuringiensis and accumulated as crystalline structures. B. thuringiensis transformants expressing B. sphaericus and B. thuringiensis subsp. israelensis toxins did not show a significant enhancement of toxicity against Aedes aegypti, Anopheles stephensi, and Culex pipiens larvae.