Interference between Field Excitatory Postsynaptic Potentials and Simultaneously Recorded Chronoamperometric L-Glutamate Currents in Mouse Hippocampal Slices
Interference between Field Excitatory Postsynaptic Potentials and Simultaneously Recorded Chronoamperometric L-Glutamate Currents in Mouse Hippocampal Slices
复制标题
小鼠海马切片中场兴奋性突触后电位与同时记录的计时安培 L-谷氨酸电流之间的干扰
DOI：
10.1016/j.elecom.2014.04.016
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Masao Sugawara
中科院分区：
文献类型：
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作者：
Ayumi Hirano-Iwata;Ryosuke Matsumura;Ryuta Tezuka;Michio Niwano;Tim V. P. Bliss;Masao Sugawara
在这里，我们报告了一个意想不到的相互作用场兴奋性突触后电位（fEPSP）和同时记录的计时电流法谷氨酸电流的基础上时间分辨（200 Hz）电流在小鼠海马切片。结果发现，电刺激和诱发的fEPSP诱导的电流分析电流的伪影，由于无意中形成的电流分析和fEPSP测量的电路之间的连接。使用电路仿真研究了伪影的起源。为了从观察到的电流中提取法拉第信号，我们在伪影衰减时对电流进行采样，即，刺激后450 ms。通过对谷氨酸释放概率的操作，检验了提取信号的可靠性。
Here we report on an unexpected interaction between field excitatory postsynaptic potentials (fEPSPs) and simultaneously recorded chronoamperometricl-glutamate currents based on time-resolved (200 Hz) amperometry in mouse hippocampal slices. It was found that electric stimuli and evoked fEPSPs induced an artifact on amperometric currents due to an unintentionally formed junction between the circuits of the amperometric and fEPSP measurements. The origin of the artifact was investigated using circuit simulation. To extract a faradaic signal from the observed currents, we sampled currents when the artifact decayed, i.e., 450 ms after the stimulation. The reliability of the extracted signal was examined through the manipulation ofl-glutamate release probability.