A microfluidic in vitro system for the quantitative study of the stomach mucus barrier function
A microfluidic in vitro system for the quantitative study of the stomach mucus barrier function
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DOI：
10.1039/c2lc40161d
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发表时间：
2012-01-01
期刊：
影响因子：
6.1
通讯作者：
Han, Jongyoon
中科院分区：
文献类型：
--
作者：
Li, Leon;Lieleg, Oliver;Han, Jongyoon
在胃中，一层胃粘液保护胃壁的上皮细胞免受胃腔中酸性消化液的损害。尽管进行了大量研究，但这种酸屏障的生物物理机制尚不清楚。我们提出了一种体外微流体工具来表征胃酸屏障，其中纯化的粘蛋白聚合物被“分泌”到芯片上的酸性区，模仿胃粘液在体内分泌到酸性胃腔中。该装置在体内重建H+浓度梯度和粘液层的向外流动环境。我们的实验表明，一个连续分泌的粘蛋白层阻碍酸的扩散，这表明新的见解粘蛋白的屏障作用。更广泛地说，我们的系统可以作为研究体内粘液层提供的屏障功能和研究粘液药物相互作用的平台工具。
In the stomach, a layer of gastric mucus protects the epithelial cells of the stomach wall against damage by the acidic digestive juices in the gastric lumen. Despite considerable research, the biophysical mechanisms for this acid barrier are not understood. We present an in vitro microfluidic tool to characterize the stomach acid barrier, in which purified mucin polymers are "secreted'' against an acidic zone on chip, mimicking the in vivo secretion of gastric mucus into an acidic stomach lumen. This device reconstitutes both the H+ concentration gradient and outward flow environment of the mucus layer in vivo. Our experiments demonstrate that a continuously secreted mucin layer hinders acid diffusion, suggesting novel insights into the barrier role of mucins. More broadly, our system may serve as a platform tool for studying the barrier functions provided by mucus layers in the body and for studying mucus drug interactions.