NGATHA-LIKEs Control Leaf Margin Development by Repressing CUP-SHAPED COTYLEDON2 Transcription
NGATHA-LIKEs Control Leaf Margin Development by Repressing CUP-SHAPED COTYLEDON2 Transcription
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NGATHA-LIKEs 通过抑制 CUP-SHAPED COTYLEDON2 转录来控制叶缘发育
DOI：
10.1104/pp.19.01598
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发表时间：
2020
期刊：
影响因子：
7.4
通讯作者：
Xiayan Liu
中科院分区：
文献类型：
--
作者：
Jingxia Shao;Jingjing Meng;Feng Wang;Bidong Shou;Yu Chen;Hui Xue;Jun Zhao;Yafei Qi;Lijun An;Fei Yu;Xiayan Liu
叶缘是叶形态的一个迷人的特征，有助于叶形状和形式的难以置信的多样性。杯形子叶2（CUP-SHAPED COTYLEDON 2，CUC 2）是NAM、ATAF 1，2、CUC 2（NAC）家族转录因子，作为植物器官分离和叶缘发育的中心调节因子，控制叶缘沿着锯齿的大小。CUC 2活性在转录和转录后水平受到严格调控。然而，在叶片发育过程中控制CUC 2转录的分子机制尚未完全阐明。在这里，我们报告拟南芥（拟南芥）NGATHA-LIKE 1（NGAL 1）到NGAL 3，这是三个相关的B3家族转录因子，作为叶缘锯齿形成的负调控因子。NGAL的过度表达导致“杯状”子叶和平滑的叶缘，而三重功能丧失的muglyngaltria比野生型显示出更多的锯齿状叶。RNA测序分析表明，参与叶片发育的许多转录因子基因的表达水平受到NGAL的调控，包括CUC 2。比较转录组分析进一步揭示了NGAL和CUC 2调节基因之间的显著重叠。此外，使用NGALsandCUC 2的获得和丧失功能突变体的各种组合的遗传分析证实，CUC 2在促进叶缘锯齿的形成中在NGALs的下游起作用。最后，我们证明NGAL 1直接结合到CUC 2启动子，导致抑制CUC 2表达。综上所述，NGAL 1对directCUC 2转录的抑制作用进一步表征了控制叶缘发育的调控模块。
The leaf margin is a fascinating feature of leaf morphology, contributing to the incredible diversity of leaf shapes and forms. As a central regulator of plant organ separation and margin development, CUP-SHAPED COTYLEDON2 (CUC2), a NAM, ATAF1, 2, CUC2 (NAC)-family transcription factor, governs the extent of serrations along the leaf margin. CUC2 activity is tightly regulated at transcriptional and posttranscriptional levels. However, the molecular mechanism that controlsCUC2transcription during leaf development has not been fully elucidated. Here we report that Arabidopsis (Arabidopsis thaliana) NGATHA-LIKE1 (NGAL1) to NGAL3, which are three related B3 family transcription factors, act as negative regulators of leaf margin serration formation. Over-expression ofNGALsled to “cup-shaped” cotyledons and smooth leaf margins, whereas the triple loss-of-function mutantngaltriexhibited more serrated leaves than the wild type. RNA-sequencing analyses revealed that the expression levels of a number of transcription factor genes involved in leaf development are regulated by NGALs, includingCUC2. Comparative transcriptome analyses further uncovered a significant overlap between NGAL- and CUC2-regulated genes. Moreover, genetic analyses using various combinations of gain- and loss-of-function mutants ofNGALsandCUC2confirmed that CUC2 acts downstream of NGALs in promoting the formation of leaf-margin serrations. Finally, we demonstrate that NGAL1 directly binds to theCUC2promoter causing repressedCUC2expression. In summary, directCUC2transcriptional repression by NGAL1 characterizes a further regulatory module controlling leaf margin development.