Serum phospholipid transfer protein activity and genetic variation of the PLTP gene
Serum phospholipid transfer protein activity and genetic variation of the PLTP gene
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DOI：
10.1016/s0021-9150(99)00140-9
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发表时间：
1999-09-01
期刊：
影响因子：
5.3
通讯作者：
Ehnholm, C
中科院分区：
文献类型：
--
作者：
Tahvanainen, E;Jauhiainen, M;Ehnholm, C
血清高密度脂蛋白(高密度脂蛋白)水平与冠心病风险之间的负相关已经得到了很好的证实。磷脂转移蛋白(PLTP)促进磷脂在脂蛋白之间的转移，并调节高密度脂蛋白的大小和组成。因此，它在高密度脂蛋白新陈代谢中起着核心作用。为了确定正常的PLTP血清值，对400名健康芬兰人的血清PLTP活性进行了测定。PLTP活性随年龄增长而升高(P<；0.001)，使得最年轻年龄组(25-35岁)的男性和女性的PLTP活性分别为3.81+/-0.84mU/m l/h(n=52)和3.97+/-0.11mU/m l/h(n=52)。男性为6.77±0.17mU/m l/h(n=45)，女性为6.68+/-0.15mU/m l/h(n=40)。PLTP活性与体重指数(r=0.001.2 2)、血清总胆固醇(r=0.17)、高密度脂蛋白/总胆固醇(r=-0.2 0)、甘油三酯(r=0.2 0)、载脂蛋白A-II(r=0.2 0)和γ谷氨酰转移酶(r=0.2 2)呈显著正相关(P<0.0 5)。血清PLTP活性与只含载脂蛋白A-I[Lp(A-I)颗粒]的高密度脂蛋白颗粒中载脂蛋白A-I水平呈负相关(r=-0.2，P<0.001)。检测了PLTP基因附近的-79G/T、-56G/A、-37T/C、-31A/G、Phe2Leu、Arg121Trp等6个基因内多态和2个中性多态的等位基因频率。这些基因多态与血清PLTP活性之间无显著相关性。(C)1999爱思唯尔爱尔兰科学有限公司。保留所有权利。
The inverse relationship between serum levels of high density lipoproteins (HDL) and risk of coronary heart disease is well established. The phospholipid transfer protein (PLTP) promotes the transfer of phospholipids between lipoproteins and modulates HDL size and composition. It thus plays a central role in HDL metabolism. Serum PLTP activity was measured in 400 healthy Finnish individuals in order to determine normal PLTP serum values. PLTP activity increased with age (P < 0.001), so that the PLTP activity was 3.81 +/- 0.84 mu mol/ml per h (mean +/- S.D., n = 52) for men and 3.97 +/- 0.11 mu mol/ml per h (n = 52) for women in the youngest age group (25-35 years), while it was 6.77 +/- 0.17 mu mol/ml per h (n = 45) for men and 6.68 +/- 0.15 mu mol/ml per h (n = 40) for women in the oldest age group (56-65 years). PLTP activity correlated significantly (P < 0.001) with body mass index (r = 0.22), serum total cholesterol (r = 0.17), the ratio of HDL-cholesterol/total cholesterol (r = - 0.20), triglycerides (r = 0.20), apO A-II (r = 0.20), and gamma glutamyl transferase (r = 0.22) values. Serum PLTP activity correlated negatively (r = - 0.20, P < 0.001) with levels of apolipoprotein A-I in HDL particles that contained only apo A-I [Lp(A-I) particles]. The allelic frequencies of six intragenic polymorphisms, - 79G/T, - 56G/A, - 37T/C, - 31A/G, Phe2Leu, Arg121Trp, and two neutral polymorphisms, located in the immediate vicinity of the PLTP gene were determined. There were no significant associations between these polymorphisms and serum PLTP activity. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.