Bacteria capture, lysate clearance, and plasmid DNA extraction using pH-sensitive multifunctional magnetic nanoparticles
Bacteria capture, lysate clearance, and plasmid DNA extraction using pH-sensitive multifunctional magnetic nanoparticles
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DOI：
10.1016/j.ab.2009.11.006
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发表时间：
2010-03-01
影响因子：
2.9
通讯作者：
Yang, Wanshen
中科院分区：
文献类型：
--
作者：
Shan, Zhi;Wu, Qi;Yang, Wanshen
开发了一种多功能磁性纳米颗粒 (MNP) 辅助生物分离方法，用于从大肠杆菌培养物中分离质粒 DNA (pDNA)。使用 pH 敏感的羧基修饰磁性纳米粒子，细胞捕获以及随后裂解后基因组 DNA/蛋白质复合物的去除都可以通过磁分离简单地实现。此外，通过 MNP 提取的 pDNA 的产量和纯度与使用有机溶剂或商业试剂盒获得的 pDNA 相当。这种具有时间和成本效益的方案不需要离心或沉淀步骤，并且具有自动化 DNA 提取的潜力，特别是在小型化实验室芯片应用中。 (C) 2009 Elsevier Inc. 保留所有权利。
A multifunctional magnetic nanoparticle (MNP)-assisted bioseparation method was developed to isolate plasmid DNA (pDNA) from Escherichia coli culture. Using the pH-sensitive carboxyl-modified magnetic nanoparticles, both cell capture and the subsequent removal of genomic DNA/protein complex after lysis can be achieved simply by magnetic separation. Furthermore, the yield and purity of pDNA extracted by MNPs are comparable to those obtained using organic solvents or commercial kits. This time- and cost-effective protocol does not require centrifugation or precipitation steps and has the potential for automated DNA extraction, especially within miniaturized lab chip applications. (C) 2009 Elsevier Inc. All rights reserved.