Gamma-irradiation induced changes in microbiological status, phenolic profile and antioxidant activity of peanut skin
Gamma-irradiation induced changes in microbiological status, phenolic profile and antioxidant activity of peanut skin
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DOI：
10.1016/j.jff.2014.10.034
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发表时间：
2015-01-01
影响因子：
5.6
通讯作者：
Shahidi, Fereidoon
中科院分区：
文献类型：
--
作者：
de Camargo, Adriano Costa;Bismara Regitano-d'Arce, Marisa Aparecida;Shahidi, Fereidoon
研究了γ射线辐照对花生皮中微生物生长、酚类成分和抗氧化性能的影响。5.0 kGy的伽马辐照降低了产品的微生物计数。总酚和原花青素含量，ABTS自由基阳离子，DPPH自由基，H2 O2，和羟基自由基清除能力，以及样品的还原力增加后，γ-照射在自由和不溶性结合酚馏分。然而，注意到酯化酚类物质的减少。游离酚类化合物对体外人LDL-胆固醇氧化和铜诱导的DNA链断裂的生物活性在γ-照射后得到改善。通过HPLC-DADE-ST-MSn法对酚酸类、黄酮类和原花青素进行了定性或初步鉴定，其分布顺序为游离态>酯化态>不溶结合态。原花青素二聚体A在所有酚级分中增加，而原花青素二聚体B减少。γ-辐射引起的变化可以通过分子转化、解聚和交联来解释。(C)2014爱思唯尔有限公司版权所有。
The effects of gamma-irradiation on the microbial growth, phenolic composition, and antioxidant properties of peanut skin were evaluated. Gamma-irradiation at 5.0 kGy decreased the microbiological count of the product. Total phenolic and proanthocyanidin contents, ABTS radical cation, DPPH radical, H2O2, and hydroxyl radical scavenging capacities as well as the reducing power of the sample were increased upon gamma-irradiation in both the free and insoluble-bound phenolic fractions. However, a decrease in the esterified phenolics was noticed. The bioactivity of the free phenolics against in vitro human LDL-cholesterol oxidation and copper induced DNA strand breakage was improved upon gamma-irradiation. Phenolic acids, flavonoids, and proanthocyanidins were positively or tentatively identified by HPLC-DADE-ST-MSn and their distribution was in the decreasing order of free > esterified > insoluble-bound forms. Procyanidin dimer A was increased in all phenolic fractions, whereas procyanidin dimer B decreased. Gamma-irradiation induced changes may be explained by molecular conversion, depolymerization, and cross-linking. (C) 2014 Elsevier Ltd. All rights reserved.