Comparison of Staphylococcus epidermidis isolated from prosthetic joint infections and commensal isolates in regard to antibiotic susceptibility, agr type, biofilm production, and epidemiology
Comparison of Staphylococcus epidermidis isolated from prosthetic joint infections and commensal isolates in regard to antibiotic susceptibility, agr type, biofilm production, and epidemiology
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DOI：
10.1016/j.ijmm.2012.11.001
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发表时间：
2013-01-01
影响因子：
4.1
通讯作者：
Nilsdotter-Augustinsson, Asa
中科院分区：
文献类型：
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作者：
Hellmark, Bengt;Soderquist, Bo;Nilsdotter-Augustinsson, Asa
表皮葡萄球菌是人体皮肤和浅表粘膜正常植物群中的主要菌种。然而，它也成为与异物材料相关的感染中最重要的病原体，如人工关节和心脏瓣膜。本研究的目的是为了提高。使用表型（PhenePlate系统）和基因型[多位点序列分型（MLST）]方法，在抗菌剂敏感性、agr类型、hid基因存在、生物膜产生（包括参与生物膜形成过程的伊卡和aap基因的存在）和流行病学方面，对从假体关节感染分离的表皮葡萄球菌（PJI; n = 61）和从健康个体分离的骨肉瘤（n = 24）进行比较。在PJI分离株中，大多数（67%）是多重耐药的。PJI分离株的两个主要集群可以确定; 44%属于MLST序列类型（ST）2，除一个外均为agr类型1，31%被分配为ST 215和agr类型3。在PJI分离株中，仅1株分离株为多重耐药，并且它们在分子流行病学上更加多样化，主要为MLST单例，最多3株分离株被分配至相同的ST。在41%的PJI分离株和58%的PJI分离株中检测到生物膜产生，在生物膜阳性分离株中，AAP基因（95%）比伊卡基因（62%）更频繁地被检测到。结果表明，S.分离自PJIs和Escherosal分离株的表皮葡萄球菌在抗菌药物敏感性和使用MLST的分子流行病学分型方面存在差异，但在agr类型的分布、生物膜产生或伊卡和aap基因的存在方面没有实质性差异。(C)2012 Elsevier GmbH. All rights reserved.
Staphylococcus epidermidis is the predominant bacterial species in the normal flora of the human skin and superficial mucosal membranes. However, it has also emerged as the most important pathogen in infections related to foreign-body materials, such as prosthetic joints and heart valves. The aims of this study were to characterise S. epidermidis isolated from prosthetic joint infections (PJI; n = 61) and commensal isolates from healthy individuals (n = 24) in regard to antimicrobial sensitivity, agr type, hid gene presence, biofllm production including presence of ica and aap genes involved in the biofilm formation process and epidemiology using both phenotypic (the PhenePlate-system) and genotypic [multilocus sequence typing (MLST)] methods. Among the PJI isolates, the majority (67%) were multidrug-resistant. Two major clusters of PJI isolates could be identified; 44% belonged to MLST sequence type (ST) 2, all but one were of agr type 1, and 31% were assigned ST215 and were of agr type 3. Of the commensal isolates, only one isolate was multidrug-resistant, and they were more molecular epidemiologically diverse with mainly MLST singletons and a maximum of 3 isolates assigned to the identical ST. Biofilm production was detected in 41% of the PJI isolates and 58% of the commensal isolates, with the aap gene (95%) more frequently detected than the ica genes (62%) in the biofilm-positive isolates. In conclusion, S. epidermidis isolated from PJIs and commensal isolates differed regarding antimicrobial sensitivity and molecular epidemiological typing using MLST, but not substantially in the distribution of agr types, biofilm production, or the presence of ica and aap genes. (C) 2012 Elsevier GmbH. All rights reserved.