Tetronic(®)-based composite hydrogel scaffolds seeded with rat bladder smooth muscle cells for urinary bladder tissue engineering applications.
Tetronic(®)-based composite hydrogel scaffolds seeded with rat bladder smooth muscle cells for urinary bladder tissue engineering applications.
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DOI：
10.1080/09205063.2014.989482
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Nagatomi J
中科院分区：
文献类型：
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作者：
Sivaraman S;Ostendorff R;Fleishman B;Nagatomi J
胶原蛋白等天然水凝胶为组织工程提供了理想的特性，包括细胞粘附位点，但其机械强度低，不适合膀胱组织再生。 In contrast, synthetic hydrogels such as PEG allow tuning of mechanical properties, but do not elicit protein adsorption or cell adhesion.为此，我们探索了由 Tetronic (BASF) 1107-丙烯酸酯 (T1107A) 与细胞外基质 (ECM) 部分、胶原蛋白和接种有膀胱平滑肌细胞 (BSMC) 的透明质酸相结合组成的复合水凝胶混合物的使用。 This composite hydrogel supported BSMC growth and distribution throughout the construct.与对照（无细胞）水凝胶相比，细胞水凝胶的机械性能（峰值应力、峰值应变和弹性模量）明显更高。与 BSMC 接种后 7 天的时间点相比，14 天时间点的机械测试结果表明，细胞水凝胶的极限拉伸应力（4.1 kPa 至 11.6 kPa）和弹性模量（11.8 kPa 至 42.7 kPa）显着增加。细胞结构的刚度和强度随时间的改善可归因于基质中接种的 BSMC 的连续胶原沉积和重建。 The composite hydrogel provided a biocompatible scaffold for BSMC to thrive and strengthen the matrix; furthering this trend could lead to strengthening the construct to match the mechanical properties of the bladder.
Natural hydrogels such as collagen offer desirable properties for tissue engineering, including cell adhesion sites, but their low mechanical strength is not suitable for bladder-tissue regeneration. In contrast, synthetic hydrogels such as PEG allow tuning of mechanical properties, but do not elicit protein adsorption or cell adhesion. For this reason, we explored the use of composite hydrogel blends composed of Tetronic (BASF) 1107-acrylate (T1107A) in combination with extracellular matrix (ECM) moieties collagen and hyaluronic acid seeded with bladder smooth muscle cells (BSMC). This composite hydrogel supported BSMC growth and distribution throughout the construct. When compared to the control (acellular) hydrogels, mechanical properties (peak stress, peak strain, and elastic modulus) of the cellular hydrogels were significantly greater. When compared to the 7-day time point after BSMC seeding, results of mechanical testing at the 14-day time point indicated a significant increase in both ultimate tensile stress (4.1 kPa to 11.6 kPa) and elastic modulus (11.8 kPa to 42.7 kPa) in cellular hydrogels. The time-dependent improvement in stiffness and strength of the cellular constructs can be attributed to the continuous collagen deposition and reconstruction by BSMC seeded in the matrix. The composite hydrogel provided a biocompatible scaffold for BSMC to thrive and strengthen the matrix; furthering this trend could lead to strengthening the construct to match the mechanical properties of the bladder.