Streptomycin Ototoxicity in Rat Cochlear Organotypic Cultures
Streptomycin Ototoxicity in Rat Cochlear Organotypic Cultures
复制标题
链霉素对大鼠耳蜗器官培养物的耳毒性
DOI：
10.1007/s12013-014-0273-1
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发表时间：
2015-03-01
影响因子：
2.6
通讯作者：
Dong, Pin
中科院分区：
文献类型：
--
作者：
He, Jing-chun;Han, Miao-miao;Dong, Pin
本研究旨在探讨链霉素对耳蜗毛细胞、螺旋神经节细胞和耳蜗神经纤维的耳毒性作用。培养3、4日龄F344大鼠耳蜗基底膜，用不同剂量的链霉素处理24 h，免疫荧光染色耳蜗毛细胞和螺旋神经节神经元，共聚焦显微镜下观察。毛细胞损失的增加与硫酸链霉素浓度的增加同时发生。链霉素对内部和外部毛细胞的损伤程度相似。毛细胞的损伤在根底转时比根尖转时更严重。相反，螺旋神经节神经元和听神经纤维完好无损。链霉素主要引起毛细胞的损失，但在体外对神经结构没有明显损害。链霉素诱导的耳蜗毛细胞病变始于耳蜗基部，并向耳蜗顶端延伸。体外条件下，链霉素耳毒性呈剂量依赖性。
The purpose of this study is to investigate the ototoxic effect of streptomycin on cochlear hair cells, spiral ganglion cells, and nerve fibers in cochlear organ cultures. The cochlear basilar membrane of three- or four-day-old F344 rats was cultured and treated with various doses of streptomycin for 24 h. Cochlear hair cells and the spiral ganglion neurons were stained with immunofluorescence and were observed under confocal microscope. The increase in hair cell loss was concomitant with the increase in streptomycin sulfate concentrations. Streptomycin impaired both the inner and outer hair cells at a similar degree. The damage to hair cells was more severe at basal turn than apical turn. In contrast, the spiral ganglion neurons and auditory nerve fibers were intact. Streptomycin primarily caused hair cell loss, but not significant impairment in the neural structure in vitro. Streptomycin-induced cochlear hair cell lesion was initiated at the basal turn and extended towards the apical turn. Streptomycin ototoxicity was dose-dependent under in vitro conditions.