Nutrient regulation of epothilone biosynthesis in heterologous and native production strains
Nutrient regulation of epothilone biosynthesis in heterologous and native production strains
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DOI：
10.1007/s00253-003-1263-1
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发表时间：
2003-06-01
影响因子：
5
通讯作者：
Licari, P
中科院分区：
文献类型：
--
作者：
Regentin, R;Frykman, S;Licari, P
以纤维堆囊菌（Sorangium cellulosum）和黄色粘球菌（Myxococcus xanthus）为材料，研究了不同初始浓度的铵盐和磷酸盐对埃博霉素（epothilone）生长和产量的抑制作用。埃坡霉素的产生菌S. cellulosum对铵和磷的敏感性高于异源产生菌M. xanthus在S. cellulosum中，而M. xanthus当向培养基中加入5 mM磷酸盐时，两种菌株的产量均降低60%。较高的磷酸盐浓度对M. xanthus效价，但在S.纤维素酶的活性比对照条件下低95%。还研究了向发酵培养基中补充铁的效果。在培养基中添加20 μ M铁时，两种菌株均表现出最佳产量。
Fermentation media with different initial concentrations of ammonium and phosphate salts were used to study the inhibitory effects of those ions on growth and production of epothilone in Sorangium cellulosum and Myxococcus xanthus. The native epothilone producer, S. cellulosum was more sensitive to ammonium and phosphate than the heterologous producer, M. xanthus. An ammonium concentration of 12 mM reduced epothilone titers by 90% in S. cellulosum but by only 40% in M. xanthus. When 5 mM phosphate was added to the medium, production in both strains was 60% lower. Higher phosphate concentrations had little additional effect on M. xanthus titers, but epothilone production with 17 mM extra-cellular phosphate in S. cellulosum was 95% lower than in the control condition. The effect of iron supplementation to the fermentation medium was also investigated. Both strains showed best production with 20,muM iron added to the medium.