Bmp signaling mediates endoderm pouch morphogenesis by regulating Fgf signaling in zebrafish
Bmp signaling mediates endoderm pouch morphogenesis by regulating Fgf signaling in zebrafish
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DOI：
10.1242/dev.129379
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发表时间：
2016-06-01
期刊：
影响因子：
4.6
通讯作者：
Eberhart, Johann K.
中科院分区：
文献类型：
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作者：
Ben Lovely, C.;Swartz, Mary E.;Eberhart, Johann K.
内胚层囊是一系列重复的结构，分割咽弓并帮助脊椎动物面部的图案。多种途径调节内胚层发育的复杂过程，包括骨形态发生蛋白（Bmp）途径。然而，Bmp信号在眼袋形态发生中的作用知之甚少。使用遗传和化学抑制剂的方法，我们表明，邮袋形态需要BMP信号从受精后10-18小时，紧随原肠胚。在此窗口期间阻断BMP信号传导导致囊袋和颅面骨骼的形态缺陷。使用遗传嵌合体，我们表明，BMP信号直接内胚层适当的形态发生。延时成像和报告转基因的分析表明，BMP信号是必要的邮袋outpocketing通过成纤维细胞生长因子（Fgf）途径。双功能丧失分析表明，BMP和FGF信号相互作用协同颅面发育。总的来说，我们的分析揭示了调节脊椎动物面部发育的组织和信号相互作用。
The endodermal pouches are a series of reiterated structures that segment the pharyngeal arches and help pattern the vertebrate face. Multiple pathways regulate the complex process of endodermal development, including the Bone morphogenetic protein (Bmp) pathway. However, the role of Bmp signaling in pouch morphogenesis is poorly understood. Using genetic and chemical inhibitor approaches, we show that pouch morphogenesis requires Bmp signaling from 10-18 h post-fertilization, immediately following gastrulation. Blocking Bmp signaling during this window results in morphological defects to the pouches and craniofacial skeleton. Using genetic chimeras we show that Bmp signals directly to the endoderm for proper morphogenesis. Time-lapse imaging and analysis of reporter transgenics show that Bmp signaling is necessary for pouch outpocketing via the Fibroblast growth factor (Fgf) pathway. Double loss-of-function analyses demonstrate that Bmp and Fgf signaling interact synergistically in craniofacial development. Collectively, our analyses shed light on the tissue and signaling interactions that regulate development of the vertebrate face.